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首页> 外文期刊>Applied Microbiology >Fractionation of Dipeptidase Activities of Streptococcus lactis and Dipeptidase Specificity of Some Lactic Acid Bacteria
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Fractionation of Dipeptidase Activities of Streptococcus lactis and Dipeptidase Specificity of Some Lactic Acid Bacteria

机译：乳酸链球菌二肽酶活性的分馏和某些乳酸菌的二肽酶特异性

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Proteins in sonic extracts of Streptococcus lactis were separated by starch-gel electrophoresis at high voltage. Each slab was sliced longitudinally, and half was stained for peptidases in a mixture containing a peptide, L-amino acid oxidase (snake venom), peroxidase, and o-dianisdine; the other half was stained in amido black for protein. In addition to sonic treatment, trypsin also released enzyme from acetone-treated cells. Glycyl-L-phenylalanine, L-phenylalanyl-glycine, L-alanyl-L-phenylalanine, and L-phenylalanyl-L-alanine served as substrates in characterizing the enzymes. Five different fractions of various specificities appeared in the gels. Broad-range substrate specificities were found for sonic extracts of S. lactis, S. cremoris, S. durans, and Lactobacillus acidophilus.
机译：乳酸链球菌的超声提取物中的蛋白质通过高压淀粉凝胶电泳分离。纵向切开每块板，并在含有肽，L-氨基酸氧化酶（蛇毒），过氧化物酶和邻二苯二胺的混合物中对一半的肽酶染色。另一半在酰胺黑上染色以获得蛋白质。除声音处理外，胰蛋白酶还从丙酮处理过的细胞中释放酶。甘氨酰-L-苯丙氨酸，L-苯丙氨酰基-甘氨酸，L-丙氨酰基-L-苯丙氨酸和L-苯丙氨酰基-L-丙氨酸用作表征酶的底物。各种特异性的五个不同部分出现在凝胶中。发现乳酸链球菌，克雷莫氏链球菌，杜氏链球菌和嗜酸乳杆菌的声音提取物具有广泛的底物特异性。

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