Reassessment of the Coagulase and Thermortable Nuclease Tests as Means of Identifying Staphylococcus aureus

M. K. Rayman; C. E. Park; J. Philpott; E. C. D. Todd

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Reassessment of the Coagulase and Thermortable Nuclease Tests as Means of Identifying Staphylococcus aureus

机译：重新评估凝血酶和耐热核酸酶测试作为鉴定金黄色葡萄球菌的手段

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A total of 91 enterotoxigenic strains of Staphylococcus aureus isolated from foods and tested for production of coagulase and thermostable nuclease and the ability to ferment glucose and mannitol showed, with the exception of four strains, a complete correlation among these properties. A similar correlation was observed with 103 cultures of S. aureus isolated from clinical material. In all instances, the coagulase reactions were sufficiently strong to be scored at either the 3+ or 4+ levels. Presumptive staphylococcal cultures isolated during routine examination of foods and yielding 2+ coagulase reactions or lower were invariably negative for themostable nuclease production. It is suggested that the thermostable nuclease test be performed on cultures with doubtful coagulase reactions before classifying them as S. aureus.

机译：从食品中分离出的共91株产肠毒素的金黄色葡萄球菌菌株经测试可产生凝结酶和热稳定核酸酶，并具有发酵葡萄糖和甘露醇的能力，除四种菌株外，这些特性之间具有完全的相关性。从临床材料中分离出的103种金黄色葡萄球菌培养物也观察到类似的相关性。在所有情况下，凝固酶反应都足够强，可以在3+或4+水平上评分。在食品的常规检查过程中分离出的葡萄球菌推定培养物，并产生2+或更低的凝结酶反应，对于最稳定的核酸酶生产而言，始终是阴性的。建议对凝固酶反应不确定的培养物进行热稳定核酸酶测试，然后将其分类为金黄色葡萄球菌。

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《Applied Microbiology》 |1975年第4期|共4页
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M. K. Rayman; C. E. Park; J. Philpott; E. C. D. Todd;
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中图分类微生物学;
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机译：常规药敏试验，青霉素结合蛋白2a乳胶凝集试验和mecA实时PCR用于检测金黄色葡萄球菌和凝固酶阴性葡萄球菌对奥沙西林耐药性的比较
2. Differentiation Between Staphylococcus Aureus and Coagulase-Negative Staphylococcus Species by Real-Time PCR Including Detection of Methicillin Resistants in Comparison to Conventional Microbiology Testing [J] . Klaschik Sven, Lehmann Lutz E., Steinhagen Folkert, Journal of clinical laboratory analysis. . 2015,第2期

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3. Differentiation Between Staphylococcus Aureus and Coagulase‐Negative Staphylococcus Species by Real‐Time PCR Including Detection of Methicillin Resistants in Comparison to Conventional Microbiology Testing [J] . Sven Klaschik, Lutz E. Lehmann, Folkert Steinhagen, Journal of clinical laboratory analysis. . 2015,第2期

机译：实时荧光定量PCR鉴别金黄色葡萄球菌和凝固酶阴性葡萄球菌种类，包括与常规微生物学检测方法比较，检测耐甲氧西林的能力
4. Coagulase Negative Staphylococci Distribution in Dairy Herds with Automatic Milking System and Their Crosstalk with Staphylococcus aureus from Intramammary Infections and Teat Apex [C] . Annual Meeting of The National Mastitis Council (U.S.). . 2018

机译：凝固酶阴性葡萄球菌分布在乳制品牛群中，自动挤奶系统及其串扰于脑内感染和奶嘴的金黄色葡萄球菌
5. An evaluation of Staphylococcus aureus knowledge and information processing for herd and cow-level decision-making: (a) Staphylococcus aureus-specific antibody test. (b) Processing diagnostic test information. (c) Literature review of Staphylococcus aureus-specific knowledge: (i) Cow-level diagnostic tests. (ii) Herd-level diagnostic tests. (iii) Decision dynamics and efficacy [D] . Costello, Sandra Swindell 1998

机译：评价金黄色葡萄球菌在牛群和奶牛一级决策中的知识和信息处理：（a）金黄色葡萄球菌特异性抗体测试。（b）处理诊断测试信息。（c）有关金黄色葡萄球菌特定知识的文献综述：（i）母牛水平的诊断测试。（ii）牧群水平的诊断测试。（iii）决策动态和效力
6. Reassessment of the Coagulase and Thermortable Nuclease Tests as Means of Identifying Staphylococcus aureus [O] . M. K. Rayman, C. E. Park, J. Philpott, 1975

机译：重新评估凝血酶和耐高温核酸酶测试作为鉴定金黄色葡萄球菌的手段
7. Reassessment of the Coagulase and Thermortable Nuclease Tests as Means of Identifying Staphylococcus aureus [O] . M. K. Rayman, C. E. Park, J. Philpott, 1975

机译：重新评估凝固酶和热量核酸酶试验作为鉴定金黄色葡萄球菌的方法

Reassessment of the Coagulase and Thermortable Nuclease Tests as Means of Identifying Staphylococcus aureus

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