Simple Process for the Reduction in the Nucleic Acid Content in Yeast

J. A. Zee; R. E. Simard

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Simple Process for the Reduction in the Nucleic Acid Content in Yeast

机译：降低酵母中核酸含量的简单方法

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A simple one-step process for the nucleic acid reduction in Rhodotorula glutinis is described. The process consists of submitting the yeast cells to a heat treatment in an acidic (pH 2) spent medium. The optimal temperature for pH 2 medium is 90 C and the final nucleic acid content in treated yeasts was 1.2%. Heat treatment at acidic pH is preferred to that at alkaline pH because it offers a better protection for amino acids and crude protein, while being more efficient in lowering the nucleic acid level. The new process is economic and rapid and could be easily used for industrial application.

机译：描述了一种简单的一步法，用于减少谷氨酸红假单胞菌中的核酸。该过程包括使酵母细胞在酸性（pH 2）用过的培养基中进行热处理。 pH 2培养基的最佳温度为90°C，处理过的酵母中的最终核酸含量为1.2％。在酸性pH下的热处理比在碱性pH下的热处理更好，因为它可以更好地保护氨基酸和粗蛋白，同时更有效地降低核酸水平。该新方法经济且迅速，可以很容易地用于工业应用。

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《Applied Microbiology》 |1975年第1期|共4页
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J. A. Zee; R. E. Simard;
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1. Reduction of Nucleic Acid Content in Candida Yeast Cells by Bovine Pancreatic Ribonuclease A Treatment [J] . A. C. Castro, A. J. Sinskey, S. R. Tannenbaum Applied Microbiology . 1971,第3期

机译：牛胰核糖核酸酶A处理降低念珠菌酵母细胞中的核酸含量
2. Characterization of a Heat-Shock Process for Reduction of the Nucleic Acid Content of Candida utilis [J] . S. Ohta, S. Maul, A. J. Sinskey, Applied Microbiology . 1971,第3期

机译：减少Process鱼假丝酵母核酸含量的热激过程的表征
3. ON METHODS FOR REDUCTION OF NUCLEIC ACIDS CONTENT IN A SINGLE-CELL PROTEIN FROM GAS OIL [J] . Abouzeid AZA., Abulnaja KO., Khan JA. Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 1995,第1期

机译：气中还原单细胞蛋白质中核酸含量的方法研究
4. Strategies for automated sample preparation nucleic acid purification and concentration of low-target-number nucleic acids in environmental and food processing samples [C] . Cynthia J. Bruckner-Lea, David A. Holman, Beatrice L. Schuck, Conference on pathogen detection and remediation for safe eating . 1999

机译：自动样品制备核酸纯化和环境和食品加工样品中低靶数核酸浓度的策略
5. The yeast STM1 protein binds G*G multiplex nucleic acids in vitro and associates with ribosomes in vivo. [D] . Nelson, Laura Diane. 2002

机译：酵母STM1蛋白在体外与G * G多重核酸结合，并在体内与核糖体缔合。
6. Simple Process for the Reduction in the Nucleic Acid Content in Yeast [O] . J. A. Zee, R. E. Simard 1975

机译：降低酵母中核酸含量的简单方法
7. Simple Process for the Reduction in the Nucleic Acid Content in Yeast [O] . J. A. Zee, R. E. Simard 1975

机译：减少酵母中核酸含量的简单方法
8. Selective Reductions. 39. Partial Reduction of Carboxylic Acids with Thexylchloroborane-Methyl Sulfide. A Direct and Simple Aldehyde Synthesis [R] . Brown, H. C., Cha, J. S., Yoon, N. M., 1987

机译：选择性减少。 39.用氯代硼烷 - 甲基硫醚部分还原羧酸。直接和简单的醛合成

Simple Process for the Reduction in the Nucleic Acid Content in Yeast

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