Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes

F. Joseph Pollock; Pamela J. Morris; Bette L. Willis; David G. Bourne

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Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes

机译：TaqMan荧光探针实时荧光定量PCR检测和鉴定珊瑚病原性珊瑚弧菌

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A real-time quantitative PCR-based detection assay targeting the dnaJ gene (encoding heat shock protein 40) of the coral pathogen Vibrio coralliilyticus was developed. The assay is sensitive, detecting as little as 1 CFU per ml in seawater and 10~(4) CFU per cm~(2) of coral tissue. Moreover, inhibition by DNA and cells derived from bacteria other than V. coralliilyticus was minimal. This assay represents a novel approach to coral disease diagnosis that will advance the field of coral disease research.

机译：针对珊瑚病原体弧菌弧菌dnaJ基因（编码热休克蛋白40）的实时定量PCR为基础的检测方法被开发出来。该测定非常灵敏，在海水中每毫升检测到低至1 CFU，每平方厘米（2）的珊瑚组织中检测到10〜（4）CFU。而且，对DNA和除溶肠弧菌以外的细菌来源的细胞的抑制作用极小。该测定法代表了一种新颖的珊瑚病诊断方法，它将推动珊瑚病研究领域的发展。

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《Applied Microbiology》 |2010年第15期|共5页
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F. Joseph Pollock; Pamela J. Morris; Bette L. Willis; David G. Bourne;
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1. Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes [J] . F. Joseph Pollock, Pamela J. Morris, Bette L. Willis, Applied and Environmental Microbiology . 2010,第15期

机译：TaqMan荧光探针实时荧光定量PCR检测和鉴定珊瑚病原性珊瑚弧菌
2. Detection of pandemic Vibrio parahaemolyticus O3 : K6 serovar in Gulf of Mexico water and shellfish using real-time PCR with Taqman (R) fluorescent probes [J] . Rizvi AV, Panicker G, Myers ML, FEMS Microbiology Letters . 2006,第2期

机译：使用Taqman（R）荧光探针的实时PCR检测墨西哥湾水和贝类大流行性副溶血弧菌O3：K6血清型
3. Detection of Vibrio parahaemolyticus in Shellfish by Use of Multiplexed Real-Time PCR with TaqMan Fluorescent Probes [J] . Linda N. Ward, Asim K. Bej Applied and Environmental Microbiology . 2006,第3期

机译：TaqMan荧光探针多重实时荧光定量PCR检测贝类中的溶血弧菌
4. A New Readout Approach in DNA Computing Based on Real-Time PCR with TaqMan Probes [C] . Zuwairie Ibrahim, John A. Rose, Yusei Tsuboi, DNA Computing; Lecture Notes in Computer Science; 4287 . 2006

机译：TaqMan探针基于实时PCR的DNA计算中的新读出方法
5. Quorum Sensing in the Hawai`ian Coral Pathogen Vibrio coralliilyticus strain OCN008 [D] . Burger, Andrew H. 2017

机译：夏威夷珊瑚病原体弧菌弧菌OCN008的群体感应
6. Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes [O] . F. Joseph Pollock, Pamela J. Morris, Bette L. Willis, 2010

机译：TaqMan荧光探针实时PCR检测和定量分析珊瑚病原性珊瑚弧菌
7. Detection and quantification of the coral pathogen vibrio coralliilyticus by real-time PCR with TaqMan fluorescent probes [O] . Pollock, F. Joseph, Morris, Pamela J., Willis, Bette L., 2010

机译：TaqMan荧光探针实时荧光定量PCR检测和定量分析珊瑚溶菌弧菌

Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes

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