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首页> 外文期刊>Applied Microbiology >Interplay between Iron Homeostasis and the Osmotic Stress Response in the Halophilic Bacterium Chromohalobacter salexigens
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Interplay between Iron Homeostasis and the Osmotic Stress Response in the Halophilic Bacterium Chromohalobacter salexigens

机译：嗜盐细菌嗜盐细菌salexigens中铁稳态与渗透应激反应之间的相互作用

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In this study, the connection between iron homeostasis and the osmostress response in the halophile Chromohalobacter salexigens was investigated. A decrease in the requirement for both iron and histidine and a lower level of siderophore synthesis were observed at high salinity, and these findings were correlated with a lower protein content in salt-stressed cells. A six-gene operon ( cfuABC - fur - hisI - orf6 operon) located downstream of the ectABC ectoine synthesis genes was characterized. A fur strain (in which the ferric iron uptake regulator Fur was affected) had the Mn resistance phenotype typical of fur mutants, was deregulated for siderophore production, and displayed delayed growth under iron limitation conditions, indicating that fur encodes a functional iron regulator. hisI was essential for histidine synthesis, which in turn was necessary for siderophore production. Fur boxes were found in the promoters of the cfuABC - fur - hisI - orf6 and ectABC operons, suggesting that Fur directly interacts with DNA in these regions. Fur mediated the osmoregulated inhibition of cfuABC - fur - hisI - orf6 operon expression by iron and functioned as a positive regulator of the ectABC genes under high-salinity conditions, linking the salt stress response with iron homeostasis. Excess iron led to a higher cytoplasmic hydroxyectoine content, suggesting that hydroxyectoine protects against the oxidative stress caused by iron better than ectoine. This study provides the first evidence of involvement of the iron homeostasis regulator Fur as part of the complex circuit that controls the response to osmotic stress in halophilic bacteria.
机译：在这项研究中，研究了嗜盐细菌嗜盐细菌salexigens中铁稳态与渗透压反应之间的联系。在高盐度下，铁和组氨酸的需求量减少，铁载体合成水平降低，这些发现与盐胁迫细胞中较低的蛋白质含量相关。表征了位于ectABC ectoine合成基因下游的六基因操纵子（cfuABC-fur-hisI-orf6操纵子）。具有Fur突变体典型的Mn抗性表型的Fur菌株（其中的铁铁摄取调节剂Fur受到影响），针对铁载体的产生而被去调节，并且在铁限制条件下显示出延迟的生长，表明Fur编码功能性铁调节剂。 hisI对于组氨酸合成是必不可少的，而组氨酸的合成又是铁载体的生产所必需的。在cfuABC-fur-hisI-orf6和ectABC操纵子的启动子中发现了毛发盒，表明毛发在这些区域直接与DNA相互作用。毛皮通过铁介导了对cfuABC-毛皮-hisI-orf6操纵子表达的渗透抑制作用，并在高盐度条件下充当ectABC基因的正调节剂，将盐胁迫反应与铁稳态联系起来。过量的铁导致较高的细胞质羟考胶含量，这表明羟考胶碱对铁引起的氧化应激的保护作用要好于果胶。这项研究提供了铁稳态调节器Fur参与控制嗜盐细菌对渗透压响应的复杂电路的一部分的第一个证据。

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