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首页> 外文期刊>RSC Advances >Multi-omics characterization of the osmotic stress resistance and protease activities of the halophilic bacterium Pseudoalteromonas phenolica in response to salt stress
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Multi-omics characterization of the osmotic stress resistance and protease activities of the halophilic bacterium Pseudoalteromonas phenolica in response to salt stress

机译：多OMICS表征嗜盐胁迫性磷酸盐菌酚磷酸磷酸盐的渗透胁迫性和蛋白酶活性响应于盐胁迫

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The halophilic bacterium Pseudoalteromonas phenolica is well known as a promising candidate that enables the recycling of organic wastes at high salinity. However, for industrial applications of P. phenolica further research is required to explore the biological mechanism for maximizing the activities and productivities of this bacterium. In this study, we investigated the osmotic stress resistance and specific protease activities of P. phenolica in a normal-salt medium (0.3 M NaCl) and high-salt medium (1 M NaCl) based on intra- and extracellular multi-omics approaches. Proteins related to betaine and proline biosynthesis were increased under high salt stress. The targeted metabolite analysis found that proline was overproduced and accumulated outside the cell at high salinity, and betaine was accumulated in the cell by activation of biosynthesis as well as uptake. In addition, extracellular serine proteases were shown to be upregulated in response to salt stress by the extracellular proteomic analysis. The specific proteolytic activity assay indicated that the activities of serine proteases, useful enzymes for the recycling of organic wastes, were increased remarkably under high salt stress. Our results suggest that betaine and proline are key osmoprotectant metabolites of P. phenolica , and they can be used for the improvement of protease production and P. phenolica activities for the recycling of high-salt organic wastes in the future.
机译：嗜盐细菌假酚酚酚是众所周知的候选者，其能够在高盐度下再循环有机废物。然而，对于P.P.P的工业应用，需要进一步研究，以探讨最大化该细菌的活性和生产性的生物机制。在这项研究中，我们基于内部和细胞外的多OMIC方法研究了普通盐培养基（0.3M NaCl）和高盐培养基（1M NaCl）中P.Papolica的渗透胁迫性和特定蛋白酶活性。在高盐胁迫下增加与甜菜碱和脯氨酸生物合成相关的蛋白质。靶向的代谢物分析发现，脯氨酸过度屈服并在高盐度外累积在细胞外，并且通过活化生物合成以及摄取，在细胞中积聚甜菜碱。此外，显示细胞外丝氨酸蛋白酶响应于细胞外蛋白质组学分析而上调。特定的蛋白水解活性测定表明，在高盐胁迫下显着增加了丝氨酸蛋白酶，用于再循环有机废物的有用酶的活性。我们的研究结果表明，甜菜碱和脯氨酸是P.Penolica的关键渗透剂代谢物，它们可用于改善未来高盐有机废弃物的蛋白酶生产和P.Papolica活性。

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