Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial, Plasmid, or Viral Metagenomes from an Activated Sludge Microbial Assemblage

Larissa C. Parsley; Erin J. Consuegra; Kavita S. Kakirde; Andrew M. Land; Willie F. Harper; Jr.; Mark R. Liles

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Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial, Plasmid, or Viral Metagenomes from an Activated Sludge Microbial Assemblage

机译：鉴定从活性污泥微生物集合中携带的细菌，质粒或病毒基因组的各种抗菌素耐药性决定簇

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Using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from DNA extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. Metagenomic clones and a plasmid that in Escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned DNA sequences lacking any significant homology to known antibiotic resistance determinants.

机译：使用基于序列和功能的宏基因组学方法，在宏基因组文库中鉴定了多种抗生素抗性决定簇，这些文库是从细菌染色体，质粒或活化污泥微生物组合中的病毒中提取的DNA构建的。分离了在大肠杆菌中表达对氯霉素，氨苄青霉素或卡那霉素抗性的元基因组克隆和质粒，其中许多克隆的DNA序列与已知的抗生素抗性决定簇缺乏任何显着同源性。

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《Applied Microbiology》 |2010年第11期|共5页
作者
Larissa C. Parsley; Erin J. Consuegra; Kavita S. Kakirde; Andrew M. Land; Willie F. Harper; Jr.; Mark R. Liles;
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中图分类微生物学;
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1. Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial, Plasmid, or Viral Metagenomes from an Activated Sludge Microbial Assemblage [J] . Larissa C. Parsley, Erin J. Consuegra, Kavita S. Kakirde, Applied and Environmental Microbiology . 2010,第11期

机译：鉴定从活性污泥微生物集合中携带的细菌，质粒或病毒基因组的各种抗菌素耐药性决定簇
2. Census of the Viral Metagenome within an Activated Sludge Microbial Assemblage [J] . Larissa C. Parsley, Erin J. Consuegra, Stephen J. Thomas, Applied Microbiology . 2010,第8期

机译：活性污泥微生物组合中的病毒基因组的普查。
3. Census of the Viral Metagenome within an Activated Sludge Microbial Assemblage [J] . Larissa C. Parsley, Erin J. Consuegra, Stephen J. Thomas, Applied and Environmental Microbiology . 2010,第8期

机译：活性污泥微生物组合中的病毒基因组的普查。
4. Antimicrobial Resistance and Bacterial Identification Utilizing a MicroelectronicChip Array [C] . Lorelei Westin, Carolyn Miller, James P. OConnell Water quality technology conference . 2000

机译：利用微电子芯片阵列的抗菌药耐药性和细菌鉴定
5. Discovery and Characterization of Novel Antibiotic Synthesis and Resistance Determinants from Diverse Microbial Metagenomes [D] . Parsley, Larissa Cherie 2010

机译：从不同的微生物基因组学中发现和表征新型抗生素合成和耐药性决定因素
6. Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial Plasmid or Viral Metagenomes from an Activated Sludge Microbial Assemblage [O] . Larissa C. Parsley, Erin J. Consuegra, Kavita S. Kakirde, 2010

机译：鉴定从活性污泥微生物集合中携带的细菌质粒或病毒基因组的各种抗菌素耐药性决定簇
7. Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial, Plasmid, or Viral Metagenomes from an Activated Sludge Microbial Assemblage▿ [O] . Parsley, Larissa C., Consuegra, Erin J., Kakirde, Kavita S., 2010

机译：从活化的污泥微生物组合中鉴定细菌，质粒或病毒基因组中携带的多种抗药性决定因素▿

Identification of Diverse Antimicrobial Resistance Determinants Carried on Bacterial, Plasmid, or Viral Metagenomes from an Activated Sludge Microbial Assemblage

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