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Biophysical-Immunological Assay for Ribonucleic Acid Type C Viruses

机译:C型核糖核酸病毒的生物物理-免疫学测定

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A biophysical and immunological method for characterization of ribonucleic acid type C virus suspensions is described. The method provides a relationship to the total viral mass concentration of the particle titer, the gs antigen titer, and the ultraviolet absorbance (268 nm) of 2% sodium dodecyl sulfate digests. Data for murine, rat, feline, and hamster viruses are shown to be analogous within the test limitations. From these data, an assessment of the viral purity can be made, the structural integrity can be evaluated, an approximate molecular weight can be computed, and the mole ratio of gs antigen can be determined.
机译:描述了表征C型核糖核酸病毒悬浮液的生物物理和免疫学方法。该方法与颗粒滴度的总病毒质量浓度,gs抗原滴度和2%十二烷基硫酸钠消化液的紫外线吸收率(268 nm)相关。在测试范围内,鼠类,大鼠,猫和仓鼠病毒的数据显示相似。根据这些数据,可以评估病毒的纯度,可以评估结构的完整性,可以计算出近似的分子量,并且可以确定gs抗原的摩尔比。

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