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Critical-Point Drying: Rapid Method for the Determination of Bacterial Extracellular Polymer and Surface Structures

机译：临界点干燥：细菌细胞外聚合物和表面结构的快速测定方法

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摘要

The relative amount of extracellular polymer which remains about Azotobacter vinelandii, Zoogloea ramigera, Klebsiella pneumoniae, and Diplococcus pneumoniae after critical-point drying was studied by electron microscopy. The results obtained with this technique are compared to those obtained with methods that illustrate extracellular polymer, such as freeze-etching and ruthenium red staining. Comparative results indicate critical-point drying to be a rapid, reliable method for the determination of capsule-like polymer surrounding bacterial cells. In addition, critical-point drying can be used to observe morphogenetic changes, such as vesicle production.
机译：通过电子显微镜研究了在临界点干燥后残留的葡萄固氮菌，游走动杆菌，肺炎克雷伯菌和肺炎双球菌的细胞外聚合物的相对量。将该技术获得的结果与通过举例说明细胞外聚合物的方法获得的结果进行比较，例如冷冻蚀刻和钌红染色。比较结果表明，临界点干燥是确定细菌细胞周围胶囊状聚合物的一种快速，可靠的方法。此外，临界点干燥可用于观察形态发生变化，例如囊泡产生。

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