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首页> 外文期刊>Applied Microbiology >Glutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae Play a Role in Actin Dynamics through Their Trx Domains, Which Contributes to Oxidative Stress Resistance
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Glutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae Play a Role in Actin Dynamics through Their Trx Domains, Which Contributes to Oxidative Stress Resistance

机译：酿酒酵母的谷胱甘肽Grx4和Grx3通过其Trx结构域在肌动蛋白动力学中起作用，这有助于抗氧化性

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Grx3 and Grx4 are two monothiol glutaredoxins of Saccharomyces cerevisiae that have previously been characterized as regulators of Aft1 localization and therefore of iron homeostasis. In this study, we present data showing that both Grx3 and Grx4 have new roles in actin cytoskeleton remodeling and in cellular defenses against oxidative stress caused by reactive oxygen species (ROS) accumulation. The Grx4 protein plays a unique role in the maintenance of actin cable integrity, which is independent of its role in the transcriptional regulation of Aft1. Grx3 plays an additive and redundant role, in combination with Grx4, in the organization of the actin cytoskeleton, both under normal conditions and in response to external oxidative stress. Each Grx3 and Grx4 protein contains a thioredoxin domain sequence (Trx), followed by a glutaredoxin domain (Grx). We performed functional analyses of each of the two domains and characterized different functions for them. Each of the two Grx domains plays a role in ROS detoxification and cell viability. However, the Trx domain of each Grx4 and Grx3 protein acts independently of its respective Grx domain in a novel function that involves the polarization of the actin cytoskeleton, which also determines cell resistance against oxidative conditions. Finally, we present experimental evidence demonstrating that Grx4 behaves as an antioxidant protein increasing cell survival under conditions of oxidative stress.
机译：Grx3和Grx4是酿酒酵母的两种单硫醇戊二醛毒素，以前被表征为Aft1定位的调节剂，因此是铁稳态的调节剂。在这项研究中，我们目前的数据表明，Grx3和Grx4在肌动蛋白细胞骨架重塑和细胞防御中对抗由活性氧（ROS）积累引起的氧化应激具有新的作用。 Grx4蛋白在肌动蛋白电缆完整性的维持中起着独特的作用，而与它在Aft1转录调控中的作用无关。在正常条件下以及对外部氧化应激的响应中，Grx3与Grx4一起在肌动蛋白细胞骨架的组织中起累加和冗余的作用。每个Grx3和Grx4蛋白都包含一个硫氧还蛋白结构域序列（Trx），其后是一个戊二醛毒素结构域（Grx）。我们对两个域分别进行了功能分析，并为它们描述了不同的功能。两个Grx结构域中的每一个都在ROS解毒和细胞活力中起作用。但是，每个Grx4和Grx3蛋白的Trx结构域在涉及肌动蛋白细胞骨架极化的新功能中独立于其各自的Grx结构域起作用，这也决定了细胞对氧化条件的抵抗力。最后，我们提供的实验证据表明，Grx4表现为抗氧化剂蛋白，可在氧化应激条件下增加细胞存活率。

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