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首页> 外文期刊>Applied Microbiology >Quantification of Shiga Toxin-Converting Bacteriophages in Wastewater and in Fecal Samples by Real-Time Quantitative PCR
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Quantification of Shiga Toxin-Converting Bacteriophages in Wastewater and in Fecal Samples by Real-Time Quantitative PCR

机译：实时定量PCR定量分析废水和粪便中的志贺毒素转化噬菌体

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Shiga toxin-converting bacteriophages (Stx phages) are involved in the pathogenicity of some enteric bacteria, such as Escherichia coli O157:H7. Stx phages are released from their bacterial hosts after lytic induction and remain free in the environment. Samples were analyzed for the presence of free Stx phages by an experimental approach based on the use of real-time quantitative PCR (qPCR), which enables stx to be detected in the DNA from the viral fraction of each sample. A total of 150 samples, including urban raw sewage samples, wastewater samples with fecal contamination from cattle, pigs, and poultry, and fecal samples from humans and diverse animals, were used in this study. Stx phages were detected in 70.0% of urban sewage samples (10 to 10~(3) gene copies [GC] per ml) and in 94.0% of animal wastewater samples of several origins (10 to 10~(10) GC per ml). Eighty-nine percent of cattle fecal samples were positive for Stx phages (10 to 10~(5) GC per g of sample), as were 31.8% of other fecal samples of various origins (10 to 10~(4) GC per g of sample). The stx _(2) genes and stx _(2) variants were detected in the viral fraction of some of the samples after sequencing of stx _(2) fragments amplified by conventional PCR. The occurrence and abundance of Stx phages in the extraintestinal environment confirm the role of Stx phages as a reservoir of stx in the environment.
机译：志贺毒素转化噬菌体（Stx噬菌体）与某些肠细菌（如大肠杆菌O157：H7）的致病性有关。溶菌诱导后，Stx噬菌体从其细菌宿主中释放出来，并在环境中保持自由。通过基于实时定量PCR（qPCR）的实验方法分析样品中游离Stx噬菌体的存在，该方法可从每个样品的病毒级分中检测出DNA中的stx。总共使用了150个样本，包括城市原始污水样本，牛，猪和家禽粪便污染的废水样本以及人类和其他动物的粪便样本。在70.0％的城市污水样本（每毫升10至10〜（3）个基因拷贝[GC]）和94.0％的几种来源的动物废水样本中检测到Stx噬菌体（每毫升10至10〜（10）GC） 。 89％的牛粪便样本中Stx噬菌体呈阳性（每克样本10至10〜（5）GC），其他各种来源的粪便样本（每克10至10〜（4）GC）也为31.8％。的样本）。在对通过常规PCR扩增的stx_（2）片段进行测序后，在一些样品的病毒级分中检测到stx_（2）基因和stx_（2）变体。肠外环境中Stx噬菌体的出现和丰富证实了Stx噬菌体在环境中作为stx贮库的作用。

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