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Assay of Vitamins and Amino Acids With Cultured Tissue Cells and Antimetabolites

机译:培养的组织细胞和抗代谢物测定维生素和氨基酸

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A survey of growth responses of tissue-culture cells to vitamins and amino acids was undertaken to explore the potentialities of tissue culture in the assay of growth factors. An antagonist of the nutrient was included in each test system to improve its sensitivity. Addition of an antimetabolite was advantageous in the thiamine and phenylalanine assays. Tissue-culture assays of tryptophan and of phenylalanine supplemented with β-2-thienylalanine compared favorably with microbial assays, and may serve as confirmatory or supplementary test systems. The sensitivity of cultured tissue cells to minute amounts of a variety of physiologically active substances suggests their employment in hormone and toxic compound assays.
机译:对组织培养细胞对维生素和氨基酸的生长反应进行了调查,以探索组织培养在生长因子测定中的潜力。营养物的拮抗剂包含在每个测试系统中,以提高其敏感性。在硫胺素和苯丙氨酸测定中添加抗代谢物是有利的。色氨酸和补充有β-2-噻吩丙氨酸的苯丙氨酸的组织培养测定法与微生物测定法相比具有优势,并且可以用作确认或补充测试系统。培养的组织细胞对微量的各种生理活性物质的敏感性表明它们可用于激素和有毒化合物的测定。

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