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首页> 外文期刊>Applied Microbiology >Studies of Laryngotracheitis Virus in Avian Tissue Cultures III. Enhancement of Infectivity by Diethylaminoethyl-Dextran
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Studies of Laryngotracheitis Virus in Avian Tissue Cultures III. Enhancement of Infectivity by Diethylaminoethyl-Dextran

机译：鸟类组织培养中喉气管炎病毒的研究III。二乙基氨基乙基-右旋糖酐增强感染性

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摘要

Diethylaminoethyl-dextran (DEAE-D) enhanced the infectivity of laryngotracheitis virus (LTV) for chicken kidney (CK) cells when cultures were treated before inoculation with virus and when DEAE-D was present in the inoculum. Infectivity was not increased when cultures were treated after virus had adsorbed to cells; since infection was not synchronized, most of the virus had probably already penetrated the plasma membrane by the time DEAE-D was added. Maximal enhancement occurred when DEAE-D was present in the inoculum. Enhancement of a lesser degree occurred when virus and DEAE-D were mixed, diluted, and inoculated onto cultures. Adsorption of LTV at 37 C as compared to that at 5 C usually yields about a threefold greater number of plaques after a 2-hr adsorption period. However, when DEAE-D was incorporated in the inoculum, greater enhancement occurred at 5 C than at 37 C, and the number of plaques produced at both adsorption temperatures was about equal. Results are compatible with the hypothesis that increased adsorption is a factor in enhancement of infectivity of LTV by DEAE-D.
机译：当在接种病毒之前对培养物进行处理并且接种物中存在DEAE-D时，二乙氨基乙基葡聚糖（DEAE-D）会增强喉气管炎病毒（LTV）对鸡肾（CK）细胞的感染性。当病毒吸附到细胞后处理培养物时，感染力没有增加;由于感染是不同步的，因此在添加DEAE-D时，大多数病毒可能已经穿透了质膜。当接种物中存在DEAE-D时，最大程度增强。当将病毒和DEAE-D混合，稀释并接种到培养物中时，发生的增强程度较小。在2小时的吸附时间后，与5 C相比，在37 C下LTV的吸附通常会产生大约三倍的噬菌斑数量。然而，当将DEAE-D掺入接种物中时，在5℃下比在37℃下发生更大的增强，并且在两个吸附温度下产生的噬菌斑数量大约相等。结果与以下假设相吻合：吸附增加是DEAE-D增强LTV感染力的一个因素。

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