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Microagglutination Procedures for Febrile Agglutination Tests

机译：微凝集程序用于高热凝集试验

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Febrile agglutination tests were done by using as antigens Brucella abortus, Salmonella group D, Proteus OX19, and Pasteurella tularensis. Comparison of results from 23 sera showed that the microtechnique, rapid slide, and test tube methods gave similar titers, although those from the microtechnique were generally higher. The sensitivity of the microtechnique depended upon the concentration of antigen, and, to obtain reproducible results, the optimal concentration of antigens had to be determined by preliminary titrations against specific, positive control antisera. Readability of reactions in the microtechnique was enhanced by adding the dye Safranin O to diluent for antigen and by use of V-type, rather than U-type, microtiter plates. Tests were also done to determine the effects of dye and salt concentrations, pH, and temperature of incubation upon the titer of agglutinations by the microtechnique. Our results indicated that the microtechnique could be used for agglutination tests involving febrile antigens. The procedure is less time-consuming than the tube method and requires less antigen and serum than the latter method or the rapid slide method.
机译：通过使用流产布鲁氏菌，沙门氏菌D组，变形杆菌OX19和图氏巴斯德氏菌作为抗原进行高热凝集试验。来自23个血清的结果的比较显示，尽管来自微技术的那些通常更高，但微技术，快速滑动和试管方法的滴度相似。微技术的敏感性取决于抗原的浓度，为了获得可再现的结果，必须通过针对特异性阳性对照抗血清的初步滴定来确定抗原的最佳浓度。通过向染料稀释液中添加染料红S染料O以及使用V型而非U型微量滴定板，可以提高微技术中反应的可读性。还进行了测试，以确定染料和盐浓度，pH值和孵育温度对通过微技术凝集的滴度的影响。我们的结果表明，该微技术可用于涉及发热抗原的凝集试验。与后一种方法或快速玻片方法相比，该方法比试管法耗时少，所需的抗原和血清更少。

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