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首页> 外文期刊>Applied Microbiology >Morphological Observations of Diplodia maydis on Synthetic and Natural Substrates as Revealed by Scanning Electron Microscopy
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Morphological Observations of Diplodia maydis on Synthetic and Natural Substrates as Revealed by Scanning Electron Microscopy

机译：扫描电子显微镜揭示的双翅目在合成和天然基质上的形态学观察

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Mycelial and spore morphology of Diplodia maydis were investigated by using scanning electron microscopy after growth on various media and natural substrates (oat and corn kernels, and corn husks). Of several specimen preparation methods studied, Parducz fixation followed by critical-point or freeze-drying gave adequate preservation for pycnidia, mycelia, and spores. Morphological characteristics were similar in rotary and reciprocal shaker cultures and differed from that found in stationary cultures in the amount of slime-like material produced and precipitated matter on the mycelial surfaces. In general, mycelial surfaces were smooth. Large areas of coalesced material were present in all samples examined. Slime-like material produced in liquid media appeared as a finely laced net, randomly appearing throughout the mycelia with bead-like structures present along the net. A fine netting also was observed interspersed among the spores inside the pycnidia obtained from oats. Slime-like material was observed to cover the pycnidia produced on oat and corn kernels. In the latter case, the spores were less protected by the outer slime-like covering. Thickened node-like structures were observed in mycelial mats produced in modified Fries 2 medium, on potato dextrose agar plates, and on infected oats. Round and ovate thickened node-like structures were observed in mycelium produced on corn kernels. In general, node-like structures were less abundant in mycelia from naturally infected substrates. Conidia were commonly rounded to tapered and two celled, with a distinctive ridged septum at the middle. Dried spores were collapsed in a characteristic flask-like fashion.
机译：在多种培养基和天然底物（燕麦和玉米粒以及玉米壳）上生长后，可通过扫描电子显微镜研究双翅目的菌丝体和孢子形态。在研究的几种标本制备方法中，先进行Parducz固定，然后进行临界点或冷冻干燥，即可对pycnidia，菌丝体和孢子进行充分的保存。旋转和往复振动培养的形态特征相似，与固定培养的形态特征不同，其产生的粘液状物质的数量和在菌丝体表面上沉淀的物质。通常，菌丝体表面是光滑的。所有检查的样品中均存在大面积的聚结材料。在液体培养基中产生的粘液状物质表现为细条状的网状，在整个菌丝体中随机出现，沿网状存在珠状结构。在从燕麦获得的伪胞内的孢子间还散布着细网。观察到粘液状物质覆盖了燕麦和玉米粒产生的孢子虫。在后一种情况下，孢子受到粘液状外层的保护较少。在改良的Fries 2培养基，马铃薯葡萄糖琼脂平板和受感染的燕麦上产生的菌丝体垫中观察到增厚的节状结构。在玉米粒上产生的菌丝体中观察到圆形和卵形的增厚的节状结构。通常，从自然感染的基质中，菌丝体中的节状结构较少。分生孢子通常被弄圆成圆锥形，两个细胞，中间有一个独特的脊状隔膜。干燥的孢子以典型的烧瓶状方式塌陷。

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