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首页> 外文期刊>Applied Microbiology >Bacterial Content in Samples from Different Sites in the Rumen of Sheep and Cows as Determined in Two Culture Media
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Bacterial Content in Samples from Different Sites in the Rumen of Sheep and Cows as Determined in Two Culture Media

机译：两种培养基测定的羊，牛瘤胃不同部位样品中的细菌含量

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Samples of material from the rumen of cattle and of sheep were cultured in both Gall Broth (GB) and Reinforced Clostridium Medium (RCM). Samples were taken, at monthly intervals, from five sites in cattle and two sites in sheep in two series and, at weekly intervals, from two sites in cattle and in sheep in two series. Comparisons were made of the growth of organisms in the media, using the number of fertile tubes per set of six dilutions as a measure of the viable count. RCM gave a greater number of fertile tubes than GB with samples from cattle, but the reverse was the case with samples from sheep. Real differences in the number of fertile tubes from different sites in cattle were found at different times, but there was no consistent pattern. Simultaneous sampling from different sites in the rumen is, therefore, essential to provide an estimate of sampling error within animals. Although no site to site differences were detectable with rumen material from sheep, it is advisable to take more than one sample at a given time to assess sampling error.
机译：在胆肉汤（GB）和增强梭状芽孢杆菌培养基（RCM）中培养牛和绵羊瘤胃的材料样品。每月从牛的五个地点和绵羊的两个地点中抽取两个系列的样品，每周一次，从牛的两个地点和绵羊中的两个系列中抽取样品。使用每组六个稀释液中可育管的数量作为活菌计数的方法，对培养基中生物的生长进行了比较。与来自牛的样品相比，RCM的可育管数量要比GB的多，但来自绵羊的样品则相反。在不同的时间发现了来自牛不同部位的可育管的数量的实际差异，但没有一致的规律。因此，从瘤胃不同部位同时取样对于估算动物内部的取样误差至关重要。尽管用羊的瘤胃材料检测不到站点间的差异，但建议在给定的时间采集多个样本以评估采样误差。

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