Production and Partial Purification of Antibiotic Materials Formed by Physarum gyrosum

Judith M. Considine; M. F. Mallette

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Production and Partial Purification of Antibiotic Materials Formed by Physarum gyrosum

机译：草形成的抗生素材料的生产和部分纯化

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Pure cultures of Physarum gyrosum were grown on agar plates with autoclaved Escherichia coli suspensions as the growth medium. Portions of such agar, after growth of the slime mold, contained diffusible materials that inhibited the growth of Bacillus subtilis, B. cereus, E. coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Paper chromatography of extracts of such cultures revealed at least three different active fractions. Preliminary fractionations increased the specific activity by one order of magnitude, probably in part by removal of inactive material and in part by separating active components. The fractionations also demonstrated the multiplicity of the antibiotic activity. Fractions variously obtained always retarded the growth of the bacterial species listed above.

机译：在高压灭菌的大肠杆菌悬浮液作为生长培养基的琼脂平板上培养纯的Physarum gyrosum培养物。粘菜霉菌生长后，这些琼脂的部分含有可扩散物质，这些物质可抑制枯草芽孢杆菌，蜡状芽孢杆菌，大肠杆菌，金黄色葡萄球菌和铜绿假单胞菌的生长。这种培养物的提取物的纸层析显示至少三种不同的活性级分。初步分馏将比活提高了一个数量级，可能部分是通过去除非活性物质，部分是通过分离活性成分。分馏还证明了抗生素活性的多样性。各种获得的馏分总是阻碍上述细菌种类的生长。

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《Applied Microbiology》 |1965年第3期|共5页
作者
Judith M. Considine; M. F. Mallette;
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中图分类微生物学;
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6. Production and Partial Purification of Antibiotic Materials Formed by Physarum gyrosum [O] . Judith M. Considine, M. F. Mallette 1965

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7. Isolation and Purification of Antibiotic Material from Physarum gyrosum [O] . Schroeder, H. R., Mallette, M. F. 1973

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Production and Partial Purification of Antibiotic Materials Formed by Physarum gyrosum

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