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Fermentative Production of Exocellular Glucans by Fleshy Fungi

机译:肉质真菌发酵生产胞外葡聚糖

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Two specimens of higher fungi produced exocellular β-1, 3-glucans when their mycelial forms were cultivated under submerged aerobic conditions. Plectania occidentalis NRRL 3137 consumed up to 6% glucose or xylose with about 30% conversion to polymer in a medium composed of hydrolyzed soy protein, salts, and thiamine. A 5% inoculum was used in a 10-day shaken fermentation. After dilution of the culture liquors and partial disruption of mycelia with a blender, solids were removed by centrifugation, and the polymer was precipitated by the admixture of 2 volumes of ethyl alcohol. A second polymer was formed in 40 to 65% yield by fermentation with Helotium sp. NRRL 3129, which in the imperfect stage would be identified as Monilia sp. It consumed up to 4% glucose, fructose, mannose, or sucrose in 60 to 72 hr. A 2% inoculum in a medium composed of commercial defatted soy flakes, phosphate, and thiamine in tap water gave a satisfactory fermentation. This polymer was precipitated by the addition of 0.5 volume of ethyl alcohol. Both organisms have a broad pH optimum on the slightly acidic side and did best at about 25 C.
机译:当在水下好氧条件下培养菌丝体时,两个高等真菌标本产生胞外β-1、3-葡聚糖。 Plectania occidentalis NRRL 3137在由大豆蛋白,盐和硫胺素组成的介质中消耗高达6%的葡萄糖或木糖,并转化为聚合物约30%。在10天的摇动发酵中使用5%的接种物。用混合器稀释培养液并部分破坏菌丝体后,通过离心除去固体,并通过混合2体积的乙醇使聚合物沉淀。通过用Helotium sp。发酵以40-65%的收率形成第二种聚合物。 NRRL 3129,在不完善的阶段将被识别为Monilia sp.。它在60到72小时内消耗了高达4%的葡萄糖,果糖,甘露糖或蔗糖。在自来水中由商业脱脂大豆薄片,磷酸盐和硫胺素组成的培养基中接种2%的菌剂,发酵效果令人满意。通过加入0.5体积的乙醇使该聚合物沉淀。两种生物在弱酸性方面均具有最适的pH最佳值,并且在约25°C时表现最佳。

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