...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Production of Tumor-Inhibitory L-Asparaginase by Submerged Growth of Serratia marcescens
【24h】

Production of Tumor-Inhibitory L-Asparaginase by Submerged Growth of Serratia marcescens

机译：粘质沙雷氏菌的水下生长产生抑制肿瘤的L-天冬酰胺酶

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Production of a tumor-inhibitory asparaginase by submerged fermentation with Serratia marcescens ATCC 60 was studied to ascertain optimal nutritional conditions for large-scale production leading to enzyme purification studies. Five strains of S. marcescens were screened in shake-flask studies and were found to produce 0.8 to 3.7 IU/ml 48 hr after inoculation. The requirements for asparaginase production with S. marcescens ATCC 60, the high producing strain, included the following: 4% autolyzed yeast extract medium (initial pH 5.0), an incubation temperature of 26 C, and limited aeration for a zero level of dissolved oxygen during the fermentation. Addition of various carbohydrates to the fermentation medium did not enhance yields. The peak cell population in the fermentation medium and the maximal asparaginase yields occurred simultaneously. Highest enzyme yields were found when the pH of the fermentation cycle rose to approximately 8.5. Yields of 4 IU of asparaginase/ml of cell suspension have been obtained consistently in 40 to 42 hr from 10-liter volumes (500 ml/4-liter bottle) produced on a reciprocating shaker. Scale-up to a 60-liter fermentor yielded 3.1 IU/ml in 35 hr.
机译：研究了通过粘质沙雷氏菌ATCC 60的深层发酵生产抑制肿瘤的天冬酰胺酶，以确定大规模生产的最佳营养条件，从而进行了酶的纯化研究。在摇瓶研究中筛选了五株粘质沙雷氏菌，发现它们在接种后48小时产生0.8至3.7 IU / ml。用生产高产的marcescences marcescens ATCC 60生产天冬酰胺酶的要求包括：4％的自溶酵母提取培养基（初始pH 5.0），温育温度为26°C和有限通气以实现零溶解水平发酵过程中的氧气。向发酵培养基中添加各种碳水化合物并没有提高产量。发酵培养基中的峰值细胞群体和最大的天冬酰胺酶产量同时发生。当发酵周期的pH升至约8.5时，发现酶的产量最高。从往复式振荡器上产生的10升体积（500毫升/ 4升瓶）中，在40至42小时内始终获得4 IU天冬酰胺酶/ ml细胞悬液的产量。按比例放大至60升发酵罐可在35小时内产生3.1 IU / ml。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号