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首页> 外文期刊>Applied Microbiology >Failure to Detect Cell-Associated Enterotoxin B in Staphylococcus aureus by Immunofluorescence
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Failure to Detect Cell-Associated Enterotoxin B in Staphylococcus aureus by Immunofluorescence

机译：未能通过免疫荧光检测金黄色葡萄球菌中的细胞相关肠毒素B

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摘要

Enterotoxin B-producing and -nonproducing Staphylococcus aureus strains showed cell fluorescence when tested with fluoresceinisothiocyanate-labeled rabbit anti-enterotoxin B globulin, probably as a result of a protein A-immunoglobulin G (Ig G) interaction. No cell-bound enterotoxin B could be detected by immunofluorescence using F(ab1)2-fragments of anti-enterotoxin B globulin. However, soluble enterotoxin B could be estimated by immunofluorescence. Approximately 1,000-fold more enterotoxin B was detected by immunodiffusion as an extracellular product in the media than could be detected in the cell fraction. The results show that intact Ig G is not suitable for the detection of antigens other than protein A on the cell surface of S. aureus in conventional immunofluorescence. For such purposes, the use of F(ab1)2-fragments of Ig G is recommended.
机译：当用异硫氰酸荧光素标记的兔抗肠毒素B球蛋白测试时，产生和不产生肠毒素B的金黄色葡萄球菌菌株显示细胞荧光，这可能是蛋白A-免疫球蛋白G（Ig G）相互作用的结果。使用抗肠毒素B球蛋白的F（ab1）2片段进行免疫荧光检测，无法检测到细胞结合的肠毒素B。但是，可通过免疫荧光法评估可溶性肠毒素B。通过免疫扩散在培养基中作为细胞外产物检测到的肠毒素B比在细胞级分中检测到的多大约1,000倍。结果表明，在常规免疫荧光法中，完整的Ig G不适合检测金黄色葡萄球菌细胞表面蛋白A以外的抗原。为此，建议使用Ig G的F（ab1）2片段。

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