Assay Methods for Clostridium perfringens Type A Enterotoxin

C. Genigeorgis; G. Sakaguchi; H. Riemann

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Assay Methods for Clostridium perfringens Type A Enterotoxin

机译：产气荚膜梭菌A型肠毒素的测定方法

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Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sensitive test, followed by microslide diffusion, single gel diffusion and electroimmunodiffusion, guinea pig skin test, mouse test, and rabbit ileal loop test.

机译：通过产气荚膜梭状芽孢杆菌NCTC 8798的孢子培养物产生的肠毒素被纯化至每毫克氮3,500小鼠平均致死剂量。高滴度血清是从注射了肠毒素的兔身上获得的，用于比较血清学检测和产气荚膜梭菌肠毒素的生物测定的敏感性。迄今为止，逆向被动血凝试验是最敏感的测试，其次是微玻片扩散，单凝胶扩散和电免疫扩散，豚鼠皮肤测试，小鼠测试和兔回肠loop回测试。

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《Applied Microbiology》 |1973年第1期|共5页
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C. Genigeorgis; G. Sakaguchi; H. Riemann;
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1. Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes [J] . Qiyi Wen, Kazuaki Miyamoto, Bruce A. McClane Journal of Clinical Microbiology . 2003,第4期

机译：用于区分产气荚膜梭状芽孢杆菌A型分离株携带染色体肠毒素（cpe）基因的双重PCR基因分型方法的开发
2. Multiplex PCR Genotyping Assay That Distinguishes between Isolates of Clostridium perfringens Type A Carrying a Chromosomal Enterotoxin Gene (cpe) Locus, a Plasmid cpe Locus with an IS1470-Like Sequence, or a Plasmid cpe Locus with an IS1151 Sequence [J] . Kazuaki Miyamoto, Qiyi Wen, Bruce A. McClane Journal of Clinical Microbiology . 2004,第4期

机译：多重PCR基因分型分析，可区分携带细菌肠毒素基因（cpe）基因座，具有IS1470序列的质粒cpe基因座或具有IS1151序列的质粒cpe基因座的产气荚膜梭状芽孢杆菌分离株
3. Rapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay. [J] . B A McClane, R J Strouse Journal of Clinical Microbiology . 1984,第2期

机译：酶联免疫吸附法快速检测产气荚膜梭菌肠毒素。
4. Evaluation of the Ability of Di-tri-octahedral Smectite to Adhere to Clostridium difficile Toxins and Clostridium perfringens Enterotoxin In Vitro [C] . J. S. Weese, N. M. Cote, R. V. G. deGannes Annual Convention of the American Association of Equine Practitioners . 2002

机译：评价Di-Tri-octaheDral蒙脱石粘附到梭菌腹菌毒素和梭菌梭菌中的能力
5. EFFECT OF LOW-DOSE GAMMA IRRADIATION ON ENTEROTOXIN POSITIVE STRAINS OF CLOSTRIDIUM PERFRINGENS TYPE A [D] . BARNHART, HAROLD MELVIN, JR. 1976

机译：低剂量伽马射线辐照对A型梭状芽胞杆菌肠毒素阳性菌株的影响
6. Assay Methods for Clostridium perfringens Type A Enterotoxin [O] . C. Genigeorgis, G. Sakaguchi, H. Riemann 1973

机译：产气荚膜梭菌A型肠毒素的测定方法
7. Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid-Borne Enterotoxin (cpe) Genes [O] . Wen, Qiyi, Miyamoto, Kazuaki, McClane, Bruce A. 2003

机译：用于区分产气荚膜梭状芽孢杆菌A型分离株携带染色体肠毒素（cpe）基因的双重PCR基因分型方法的开发

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Assay Methods for Clostridium perfringens Type A Enterotoxin

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