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Production of Multivalent Fluorescent Antisera for Identification of Organisms in the Mycobacterium avium-Mycobacterium intracellulare Complex

机译:多价荧光抗血清的生产,用于鉴定鸟分枝杆菌-细胞内分枝杆菌复合物中的生物

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Antisera to ten strains of mycobacteria in the Mycobacterium avium-Mycobacterium intracellulare group were obtained by injecting rabbits with ultraviolet light-killed cells. The antisera were conjugated with fluorescein isothiocyanate and used in the direct fluorescent antibody test. Individual antisera reacted specifically with the mycobacterial serotype used to produce them. The antisera were then combined in two multivalent pools. Each multivalent pool reacted specifically with its corresponding antigens. The multivalent antisera were thus found to provide a rapid identification method for the mycobacteria studied.
机译:通过给兔子注射紫外线杀死的细胞,获得对鸟分枝杆菌-细胞内分枝杆菌组中十种分枝杆菌菌株的抗血清。将抗血清与异硫氰酸荧光素偶联,并用于直接荧光抗体测试。个别抗血清会与产生它们的分枝杆菌血清型发生特异性反应。然后将抗血清合并在两个多价库中。每个多价库均与其相应的抗原发生特异性反应。因此发现多价抗血清为所研究的分枝杆菌提供了快速鉴定方法。

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