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Improved Membrane Filter Method for Fecal Coliform Analysis

机译：粪便大肠菌群分析的改进膜过滤方法

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A two-layer agar method has been developed which consistently yields higher recovery of fecal coliforms on membrane filters when compared to the existing membrane fecal coliform procedure. This method has been evaluated by three laboratories using samples of raw and chlorinated waste water, and reservoir, river, and marine waters. Verification of 1,013 fecal coliform colonies isolated from 61 water samples averaged 92% on this proposed procedure. Comparison with the Standard Methods membrane fecal coliform procedure revealed the two-layer agar method had an overall increased sensitivity to fecal coliform detection in these waters. It is therefore proposed that this procedure be evaluated as an alternative to the Standard Methods fecal coliform membrane Filter test in the examination of chlorinated secondary effluents, marine waters, and any natural waters that may contain pollutants with heavy metal ions.
机译：已开发出一种两层琼脂方法，与现有的膜粪大肠菌群操作程序相比，可在膜滤器上始终产生较高的粪便大肠菌群回收率。该方法已由三个实验室使用原水和氯化废水，水库，河水和海水进行了评估。从该拟议程序中，从61个水样中分离出的1,013个粪大肠菌群的验证平均为92％。与标准方法的粪便大肠菌膜法比较表明，两层琼脂法在这些水中对粪便大肠菌群检测的灵敏度总体提高。因此，建议在评估氯化二级废水，海水和任何可能含有重金属离子污染物的天然水时，对这一程序进行评估，以替代标准方法粪便大肠菌膜过滤器测试。

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