Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

Kilian Stoecker; Christiane Dorninger; Holger Daims; Michael Wagner

首页> 外文期刊>Applied Microbiology >Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

【24h】

Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

机译：用于荧光原位杂交（DOPE-FISH）的寡核苷酸探针的双重标记可改善信号强度并增加rRNA的可及性

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Fluorescence in situ hybridization (FISH) with singly labeled rRNA-targeted oligonucleotide probes is widely applied for direct identification of microbes in the environment or in clinical specimens. Here we show that a replacement of singly labeled oligonucleotide probes with 5′-, 3′-doubly labeled probes at least doubles FISH signal intensity without causing specificity problems. Furthermore, Cy3-doubly labeled probes strongly increase in situ accessibility of rRNA target sites and thus provide more flexibility for probe design.

机译：荧光原位杂交（FISH）与单个标记的rRNA靶向寡核苷酸探针被广泛用于环境或临床标本中微生物的直接鉴定。在这里，我们显示用5'-，3'-双重标记的探针替换单标记的寡核苷酸探针至少会使FISH信号强度加倍，而不会引起特异性问题。此外，Cy3双重标记的探针极大地提高了rRNA靶位的原位可及性，因此为探针设计提供了更大的灵活性。

著录项

来源
《Applied Microbiology》 |2010年第3期|共5页
作者
Kilian Stoecker; Christiane Dorninger; Holger Daims; Michael Wagner;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类微生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility [J] . Kilian Stoecker, Christiane Dorninger, Holger Daims, Applied and Environmental Microbiology . 2010,第3期

机译：用于荧光原位杂交（DOPE-FISH）的寡核苷酸探针的双重标记可改善信号强度并增加rRNA的可及性
2. Use of Fluorochrome-Labeled rRNA Targeted Oligonucleotide Probe and Tyramide Signal Amplification to Improve Sensitivity of Fluorescence in situ Hybridization [J] . Wiwat Jupraputtasri, Supapon Cheevadhanarak, Pawinee Chaiprasert Journal of Bioscience and Bioengineering . 2004,第4期

机译：使用荧光标记的rRNA靶向的寡核苷酸探针和酪氨酰胺信号放大，以提高荧光原位杂交的敏感性
3. Unlabeled Helper Oligonucleotides Increase the In Situ Accessibility to 16S rRNA of Fluorescently Labeled Oligonucleotide Probes [J] . Bernhard M. Fuchs, Frank Oliver Gl?ckner, J?rg Wulf, Applied and Environmental Microbiology . 2000,第8期

机译：未标记的辅助寡核苷酸增加了荧光标记的寡核苷酸探针对16S rRNA的原位可达性
4. Detection of DNA hybridization with immobilized fluorescently labeled oligonucleotide probes using fluorescence polarization technique [C] . Satoshi Abe, Shuji Toyonaga Fluorescence Science and Technology . 2000

机译：用荧光偏振技术检测固定化荧光标记的寡核苷酸探针的DNA杂交
5. Studies on the relationships between oligonucleotide probe properties and hybridization signal intensities. [D] . Gharaibeh, Raad Zuhair. 2009

机译：寡核苷酸探针特性与杂交信号强度之间关系的研究。
6. Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility [O] . Kilian Stoecker, Christiane Dorninger, Holger Daims, 2010

机译：用于荧光原位杂交（DOPE-FISH）的寡核苷酸探针的双重标记可提高信号强度并增加rRNA的可及性
7. Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) improves signal intensity and increases rRNA accessibility [O] . Stöcker, Kilian, University of Vienna, Dorninger, Christiane, 2010

机译：用于荧光原位杂交（DOPE-FISH）的寡核苷酸探针的双重标记可改善信号强度并增加rRNA的可及性
8. Enumeration of 'Vibrio vulnificus' on Membrane Filters with a Fluorescently Labeled Oligonucleotide Probe Specific for Kingdom-Level 16S rRNA Sequences [R] . Heidelberg, J. F., O'Neill, K. R., Jacobs, D., 1993

机译：使用荧光标记的寡核苷酸探针对膜过滤器上的'创伤弧菌'进行计数，该探针特异于针对王国级16s rRNa序列

Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

摘要

著录项

相似文献

相关主题

期刊订阅