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首页> 外文期刊>Applied Microbiology >Involvement of Vacuolar Sequestration and Active Transport in Tolerance of Saccharomyces cerevisiae to Hop Iso-α-Acids
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Involvement of Vacuolar Sequestration and Active Transport in Tolerance of Saccharomyces cerevisiae to Hop Iso-α-Acids

机译：酿酒酵母对HopIso-α-酸的耐受性与叶片螯合和主动转运有关。

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摘要

The hop plant, Humulus lupulus L., has an exceptionally high content of secondary metabolites, the hop α-acids, which possess a range of beneficial properties, including antiseptic action. Studies performed on the mode of action of hop iso-α-acids have hitherto been restricted to lactic acid bacteria. The present study investigated molecular mechanisms of hop iso-α-acid resistance in the model eukaryote Saccharomyces cerevisiae . Growth inhibition occurred at concentrations of hop iso-α-acids that were an order of magnitude higher than those found with hop-tolerant prokaryotes. Chemostat-based transcriptome analysis and phenotype screening of the S . cerevisiae haploid gene deletion collection were used as complementary methods to screen for genes involved in hop iso-α-acid detoxification and tolerance. This screening and further analysis of deletion mutants confirmed that yeast tolerance to hop iso-α-acids involves three major processes, active proton pumping into the vacuole by the vacuolar-type ATPase to enable vacuolar sequestration of iso-α-acids and alteration of cell wall structure and, to a lesser extent, active export of iso-α-acids across the plasma membrane. Furthermore, iso-α-acids were shown to affect cellular metal homeostasis by acting as strong zinc and iron chelators.
机译：啤酒花植物Humulus lupulus L.具有特别高的次级代谢产物含量，即啤酒花α-酸，它具有一系列有益特性，包括杀菌作用。迄今为止，关于啤酒花异α-酸的作用方式的研究仅限于乳酸菌。本研究探讨了真核生物酿酒酵母模型中啤酒花异α-酸抗性的分子机制。生长抑制发生在啤酒花异α酸的浓度上，该浓度比耐啤酒花原核生物高出一个数量级。基于S的Chemostat转录组分析和表型筛选。酿酒酵母单倍体基因缺失集合被用作筛选涉及啤酒花异α-酸解毒和耐受性的基因的补充方法。缺失突变体的筛选和进一步分析证实，酵母对啤酒花异α-酸的耐受性涉及三个主要过程，即通过液泡型ATPase将活性质子泵入液泡中，以使液泡隔离异α-酸和改变细胞壁结构，并在较小程度上跨质膜主动输出异α-酸。此外，异α-酸还通过充当强锌和铁螯合剂来影响细胞金属稳态。

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