首页> 外国专利> A method for producing four or more double bond polyunsaturated fatty acids, genetically modified saccharomyces cerevisiae which is capable of producing four or more double bond polyunsaturated fatty acids when developed on a substrate other than fatty acid, composition, use of the composition and use of a genetically modified saccharomyces cervisiae

A method for producing four or more double bond polyunsaturated fatty acids, genetically modified saccharomyces cerevisiae which is capable of producing four or more double bond polyunsaturated fatty acids when developed on a substrate other than fatty acid, composition, use of the composition and use of a genetically modified saccharomyces cervisiae

机译：一种用于生产四个或多个双键多不饱和脂肪酸，转基因酿酒酵母的方法，当在脂肪酸以外的底物上显影时，该酿酒酵母能够生产四个或多个双键多不饱和脂肪酸，组合物，该组合物的用途和转基因啤酒酵母

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摘要

Method for the production of polyunsaturated fatty acids with four or more double bonds, genetically modified Saccharomyces cerevisiae which has the capacity to produce polyunsaturated fatty acids with four or more double-grained joints, Use of a Genetically Modified Saccharomyces cerevisiae The present invention relates to the construction and engineering of cells, more particularly microorganisms to produce PUFAs with four or more double bonds from non-fatty acid substrates through heterologous expression of a passageway. that requires oxygen. The invention especially involves increasing the PUFA content in the host organism by optimizing fermentation, for example by lowering the temperature and / or designing a more favorable medium or by increasing the flow towards fatty acids by metabolic engineering. for example, by overexpression of fatty acid synthases, overexpression of other enzymes involved in PUFA precursor biosynthesis, codon optimization of heterologous genes, or expression of heterologous enzymes involved in PUFA precursor biosynthesis.

机译：生产具有四个或更多个双键的多不饱和脂肪酸的方法，具有能够生产具有四个或更多个双颗粒接头的多不饱和脂肪酸的基因修饰的酿酒酵母，转基因酿酒酵母的用途本发明涉及细胞的构建和工程改造，更具体地讲是微生物，其通过通道的异源表达从非脂肪酸底物中产生具有四个或多个双键的PUFA。需要氧气。本发明尤其涉及通过优化发酵来提高宿主生物中PUFA的含量，例如通过降低温度和/或设计更有利的培养基或通过代谢工程增加向脂肪酸的流量。例如，通过脂肪酸合成酶的过表达，PUFA前体生物合成中涉及的其他酶的过表达，异源基因的密码子优化或PUFA前体生物合成中涉及的异源酶的表达。

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公开/公告号BRPI0511236A

专利类型
公开/公告日2007-11-27

原文格式PDF
申请/专利权人 FLUXOME SCIENCES A/S;
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申请/专利号BR2005PI11236
发明设计人 JOCHEN FORSTER;NINA KATARINA GUNNARSSON;JEANS BREDAL NIELSEN;
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申请日2005-06-03
分类号C12N15/52;A23L1/30;A61K8/36;A61K8/67;A61Q19;C11B1;C12N9/02;C12N9/10;C12P7/64;
国家 BR
入库时间 2022-08-21 20:08:48

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