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首页> 外文期刊>Applied Microbiology >Regulation of Cell Division, Biofilm Formation, and Virulence by FlhC in Escherichia coli O157:H7 Grown on Meat
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Regulation of Cell Division, Biofilm Formation, and Virulence by FlhC in Escherichia coli O157:H7 Grown on Meat

机译：肉中生长的大肠杆菌O157：H7中FlhC对细胞分裂，生物膜形成和毒力的调节

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To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to several cellular processes, this study compared the expression pattern of an E. coli O157:H7 parent strain to that of its isogenic flhC mutant. This was done with bacteria that had been grown on meat. Microarray experiments revealed 287 putative targets of FlhC. Real-time PCR was performed as an alternative estimate of transcription and confirmed microarray data for 13 out of 15 genes tested (87%). The confirmed genes are representative of cellular functions, such as central metabolism, cell division, biofilm formation, and pathogenicity. An additional 13 genes from the same cellular functions that had not been hypothesized as being regulated by FlhC by the microarray experiment were tested with real-time PCR and also exhibited higher expression levels in the flhC mutant than in the parent strain. Physiological experiments were performed and confirmed that FlhC reduced the cell division rate, the amount of biofilm biomass, and pathogenicity in a chicken embryo lethality model. Altogether, this study provides valuable insight into the complex regulatory network of the pathogen that enables its survival under various environmental conditions. This information may be used to develop strategies that could be used to reduce the number of cells or pathogenicity of E. coli O157:H7 on meat by interfering with the signal transduction pathways.
机译：为了了解大肠杆菌O157：H7作为食物病原体引起的持续问题，本研究评估了肉类细菌生长中的全球基因调控。由于以前已将大肠杆菌K-12实验室菌株的FlhD / FlhC作为将环境信号转导至多个细胞过程的主要控制点，因此本研究将大肠杆菌O157：H7亲本菌株的表达模式与同基因的flhC突变体。这是通过肉类上生长的细菌完成的。基因芯片实验揭示了FlhC的287个靶标。进行实时PCR作为转录的替代估计，并从测试的15个基因中的13个（87％）证实了微阵列数据。证实的基因代表细胞功能，例如中枢代谢，细胞分裂，生物膜形成和致病性。用实时PCR检测了来自相同细胞功能的另外13个基因，这些基因尚未被微阵列实验假设为受FlhC调控，并且在flhC突变体中比亲本菌株显示更高的表达水平。进行了生理学实验，并证实FlhC降低了鸡胚致死性模型中的细胞分裂速率，生物膜生物量和致病性。总而言之，这项研究为病原体的复杂调控网络提供了有价值的见解，从而使其能够在各种环境条件下生存。此信息可用于开发策略，该策略可通过干扰信号转导途径来减少肉中大肠杆菌O157：H7的细胞数或致病性。

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