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首页> 外文期刊>Applied Microbiology >Functional Analysis of the Trichoderma harzianum nox1 Gene, Encoding an NADPH Oxidase, Relates Production of Reactive Oxygen Species to Specific Biocontrol Activity against Pythium ultimum
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Functional Analysis of the Trichoderma harzianum nox1 Gene, Encoding an NADPH Oxidase, Relates Production of Reactive Oxygen Species to Specific Biocontrol Activity against Pythium ultimum

机译：编码NADPH氧化酶的哈茨木霉nox1基因的功能分析，将活性氧的产生与针对最终腐霉的特定生物防治活性相关。

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The synthesis of reactive oxygen species (ROS) is one of the first events following pathogenic interactions in eukaryotic cells, and NADPH oxidases are involved in the formation of such ROS. The nox1 gene of Trichoderma harzianum was cloned, and its role in antagonism against phytopathogens was analyzed in nox1 -overexpressed transformants. The increased levels of nox1 expression in these transformants were accompanied by an increase in ROS production during their direct confrontation with Pythium ultimum . The transformants displayed an increased hydrolytic pattern, as determined by comparing protease, cellulase, and chitinase activities with those for the wild type. In confrontation assays against P. ultimum the nox1 -overexpressed transformants were more effective than the wild type, but not in assays against Botrytis cinerea or Rhizoctonia solani . A transcriptomic analysis using a Trichoderma high-density oligonucleotide (HDO) microarray also showed that, compared to gene expression for the interaction of wild-type T. harzianum and P. ultimum , genes related to protease, cellulase, and chitinase activities were differentially upregulated in the interaction of a nox1 -overexpressed transformant with this pathogen. Our results show that nox1 is involved in T. harzianum ROS production and antagonism against P. ultimum .
机译：活性氧（ROS）的合成是在真核细胞中致病相互作用之后的首批事件之一，NADPH氧化酶参与了此类ROS的形成。克隆了哈茨木霉的nox1基因，并分析了其在过量表达nox1的转化株中对植物病原菌的拮抗作用。在这些转化子与终极腐霉菌直接对抗的过程中，nox1表达的增加伴随着ROS产生的增加。通过将蛋白酶，纤维素酶和几丁质酶活性与野生型相比，转化子显示出增加的水解模式。在对抗终极疟原虫的对抗试验中，nox1过度表达的转化体比野生型更有效，但在灰葡萄孢或番茄红枯菌的试验中却没有。使用木霉属高密度寡核苷酸（HDO）微阵列进行的转录组分析还显示，与野生型哈茨木霉和倒数第二球菌相互作用的基因表达相比，与蛋白酶，纤维素酶和几丁质酶活性有关的基因差异表达上调过量表达nox1的转化体与该病原体的相互作用。我们的结果表明，nox1参与了哈茨木霉ROS的产生和对终极草的拮抗作用。

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