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首页> 外文期刊>Applied Microbiology >Raman Spectroscopy of Xylitol Uptake and Metabolism in Gram-Positive and Gram-Negative Bacteria
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Raman Spectroscopy of Xylitol Uptake and Metabolism in Gram-Positive and Gram-Negative Bacteria

机译：革兰氏阳性细菌和革兰氏阴性细菌中木糖醇摄取和代谢的拉曼光谱

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Visible-wavelength Raman spectroscopy was used to investigate the uptake and metabolism of the five-carbon sugar alcohol xylitol by Gram-positive viridans group streptococcus and the two extensively used strains of Gram-negative Escherichia coli , E. coli C and E. coli K-12. E. coli C, but not E. coli K-12, contains a complete xylitol operon, and the viridans group streptococcus contains an incomplete xylitol operon used to metabolize the xylitol. Raman spectra from xylitol-exposed viridans group streptococcus exhibited significant changes that persisted even in progeny grown from the xylitol-exposed mother cells in a xylitol-free medium for 24 h. This behavior was not observed in the E. coli K-12. In both viridans group streptococcus and the E. coli C derivative HF4714, the metabolic intermediates are stably formed to create an anomaly in bacterial normal survival. The uptake of xylitol by Gram-positive and Gram-negative pathogens occurs even in the presence of other high-calorie sugars, and its stable integration within the bacterial cell wall may discontinue bacterial multiplication. This could be a contributing factor for the known efficacy of xylitol when taken as a prophylactic measure to prevent or reduce occurrences of persistent infection. Specifically, these bacteria are causative agents for several important diseases of children such as pneumonia, otitis media, meningitis, and dental caries. If properly explored, such an inexpensive and harmless sugar-alcohol, alone or used in conjunction with fluoride, would pave the way to an alternative preventive therapy for these childhood diseases when the causative pathogens have become resistant to modern medicines such as antibiotics and vaccine immunotherapy.
机译：使用可见光拉曼光谱研究革兰氏阳性的绿箭虫群链球菌和两种广泛使用的革兰氏阴性大肠杆菌，大肠杆菌C和大肠杆菌K菌株对五碳糖醇木糖醇的吸收和代谢。 -12。大肠杆菌C但不包含大肠杆菌K-12包含完整的木糖醇操纵子，而viridans组链球菌包含不完整的木糖醇操纵子，用于代谢木糖醇。来自木糖醇暴露的viridans组链球菌的拉曼光谱显示出显着变化，甚至在从无木糖醇培养基中暴露于木糖醇的母细胞中生长的后代中持续存在24 h。在大肠杆菌K-12中未观察到此行为。在绿豆类链球菌和大肠杆菌C衍生物HF4714中，代谢中间体稳定形成，从而在细菌正常存活中产生异常。甚至在存在其他高热量糖的情况下，革兰氏阳性和革兰氏阴性病原体也会吸收木糖醇，并且其在细菌细胞壁内的稳定整合可能会中断细菌繁殖。当采取木糖醇作为预防措施来预防或减少持续感染的发生时，这可能是木糖醇已知功效的一个促成因素。具体而言，这些细菌是儿童几种重要疾病的病原体，例如肺炎，中耳炎，脑膜炎和龋齿。如果进行了适当的研究，当致病性病原体对现代药物（例如抗生素和疫苗免疫疗法）产生耐药性时，这种廉价且无害的糖醇单独使用或与氟化物结合使用将为这些儿童疾病的替代预防方法铺平道路。

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