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Use-Dilution Test and Newcastle Disease Virus

机译：使用稀释试验和新城疫病毒

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The use-dilution test for evaluating the effectiveness of disinfectants against bacteria was modified to determine the effectiveness of disinfectants against a group of viruses. Modifications were kept to a minimum to retain the general principles of the test and thereby retain the test's familiarity among testing laboratory personnel. Modifications included the use of a standard allantoic fluid suspension of Newcastle disease virus instead of a standard bacterial culture. The only other modification was the inoculation of six embryonated chicken eggs (10 to 12 days old) with 0.1 ml of nutrient broth into which a carrier ring was transferred after a standard period in diluted disinfectant. The death or survival of 60 embryos, then, is the criterion by which a disinfectant can be judged effective at use-dilution. Experiments are described which establish the validity of the modified test procedure. The effectiveness of nine common disinfectants against Newcastle disease virus as judged by this test procedure is reported.
机译：修改了用于评估消毒剂对细菌有效性的使用稀释试验，以确定消毒剂对一组病毒的有效性。为了保持测试的一般原理，对修改进行了最小化，从而保留了测试实验室人员对测试的熟悉程度。修改包括使用新城疫病毒的标准尿囊液悬浮液代替标准细菌培养物。唯一的其他修改是用0.1 ml的营养肉汤接种6个有雏鸡的鸡蛋（10至12天大），在标准时间后将稀释的消毒液中的载体环转移到其中。因此，可以判断出60种胚胎的死亡或存活率是根据使用稀释度判断消毒剂有效的标准。描述了确定修改后的测试程序有效性的实验。据报道，通过该测试程序判断出九种常用消毒剂对新城疫病毒的有效性。

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