...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Avian Pathology >Analyses of the results of different test systems in the 2005 global proficiency testing schemes for infectious bursal disease virus and Newcastle disease virus antibody detection in chicken serum
【24h】

Analyses of the results of different test systems in the 2005 global proficiency testing schemes for infectious bursal disease virus and Newcastle disease virus antibody detection in chicken serum

机译：2005年全球鸡传染性法氏囊病病毒和新城疫病毒抗体检测全球能力验证计划中不同测试系统的结果分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The results of global proficiency testing schemes (PTS) for serological tests to detect antibodies against infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV) in chicken serum, in which 125 and 120 laboratories, respectively, participated from Africa, Asia, Europe, Central and South America, were used to analyse the performances of different antibody test systems such as virus neutralization tests, haemagglutination inhibition tests, enzyme-linked immunosorbent assays and agar gel precipitation tests. All laboratories were asked to carry out their routine diagnostic tests for the detection of IBDV and NDV antibodies as usual. This global ring trial provided a large amount of data on variation within and between laboratories and test systems used worldwide. The data showed that the variation between the quantitative test results of different laboratories (R(between)) using the IBDV virus neutralization tests and the NDV haemagglutination inhibition test was higher (about double) compared with the variation within commercial enzyme-linked immunosorbent assay systems. Although both tests are often referred to and used as the "gold standard" in experimental and scientific studies, official procedures and for the validation of tests, this study shows that there is an urgent need for a global implementation of recommended test procedures and/or the inclusion of international reference sera in these studies.
机译：全球血清学检测方案（PTS）的结果，用于血清学检测以检测鸡血清中的传染性法氏囊病病毒（IBDV）和新城疫病毒（NDV）的抗体，来自非洲，亚洲和非洲的分别有125和120个实验室参加了欧洲，中美洲和南美洲被用来分析不同抗体测试系统的性能，例如病毒中和测试，血凝抑制测试，酶联免疫吸附测试和琼脂凝胶沉淀测试。要求所有实验室像往常一样进行常规诊断测试以检测IBDV和NDV抗体。这项全球性的环试验提供了大量有关全世界使用的实验室和测试系统之间以及之间的差异的数据。数据表明，与商业酶联免疫吸附测定系统中的差异相比，使用IBDV病毒中和试验和NDV血凝抑制试验在不同实验室（R（中间））的定量试验结果之间的差异更大（约为两倍）。 。尽管这两种测试在实验和科学研究，官方程序以及测试的验证中通常都被称为“黄金标准”并用作“金标准”，但这项研究表明，迫切需要在全球范围内实施推荐的测试程序和/或在这些研究中纳入国际参考血清。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号