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首页> 外文期刊>Applied Microbiology >Genome Sequencing Identifies Two Nearly Unchanged Strains of Persistent Listeria monocytogenes Isolated at Two Different Fish Processing Plants Sampled 6 Years Apart
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Genome Sequencing Identifies Two Nearly Unchanged Strains of Persistent Listeria monocytogenes Isolated at Two Different Fish Processing Plants Sampled 6 Years Apart

机译：基因组测序确定了两个几乎不变的持久性单核细胞增生李斯特菌菌株，在两个采样间隔为6年的不同鱼类加工工厂中分离

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Listeria monocytogenes is a food-borne human-pathogenic bacterium that can cause infections with a high mortality rate. It has a remarkable ability to persist in food processing facilities. Here we report the genome sequences for two L. monocytogenes strains (N53-1 and La111) that were isolated 6 years apart from two different Danish fish processers. Both strains are of serotype 1/2a and belong to a highly persistent DNA subtype (random amplified polymorphic DNA [RAPD] type 9). We demonstrate using in silico analyses that both strains belong to the multilocus sequence typing (MLST) type ST121 that has been isolated as a persistent subtype in several European countries. The purpose of this study was to use genome analyses to identify genes or proteins that could contribute to persistence. In a genome comparison, the two persistent strains were extremely similar and collectively differed from the reference lineage II strain, EGD-e. Also, they differed markedly from a lineage I strain (F2365). On the proteome level, the two strains were almost identical, with a predicted protein homology of 99.94%, differing at only 2 proteins. No single-nucleotide polymorphism (SNP) differences were seen between the two strains; in contrast, N53-1 and La111 differed from the EGD-e reference strain by 3,942 and 3,471 SNPs, respectively. We included a persistent L. monocytogenes strain from the United States (F6854) in our comparisons. Compared to nonpersistent strains, all three persistent strains were distinguished by two genome deletions: one, of 2,472 bp, typically contains the gene for inlF , and the other, of 3,017 bp, includes three genes potentially related to bacteriocin production and transport ( lmo2774 , lmo2775 , and the 3′-terminal part of lmo2776 ). Further studies of highly persistent strains are required to determine if the absence of these genes promotes persistence. While the genome comparison did not point to a clear physiological explanation of the persistent phenotype, the remarkable similarity between the two strains indicates that subtypes with specific traits are selected for in the food processing environment and that particular genetic and physiological factors are responsible for the persistent phenotype.
机译：单核细胞增生性李斯特菌是一种食源性人类致病细菌，可引起高死亡率的感染。它具有在食品加工设施中持久的卓越能力。在这里，我们报告了与两个不同的丹麦鱼类加工商分开6年分离的两个单核细胞增生李斯特氏菌菌株（N53-1和La111）的基因组序列。两种菌株均为血清型1 / 2a，属于高度持久的DNA亚型（随机扩增多态性DNA [RAPD] 9型）。我们使用计算机分析方法证明，这两种菌株均属于多位点序列类型（MLST）类型ST121，已在多个欧洲国家中作为持久亚型分离出来。这项研究的目的是使用基因组分析来鉴定可能有助于持久性的基因或蛋白质。在基因组比较中，这两个持久株与参考谱系II株EGD-e极为相似且总体不同。而且，它们与I谱系（F2365）明显不同。在蛋白质组水平上，两个菌株几乎相同，预测的蛋白质同源性为99.94％，仅两个蛋白质不同。在两个菌株之间没有发现单核苷酸多态性（SNP）差异。相反，N53-1和La111与EGD-e参考菌株的差异分别为3,942和3,471个SNP。在比较中，我们包括了来自美国的持久性单核细胞增生李斯特菌菌株（F6854）。与非持久性菌株相比，所有三个持久性菌株的特征均在于两个基因组缺失：一个是2,472 bp，通常包含inlF基因，另一个是3,017 bp，包括三个可能与细菌素生产和运输有关的基因（lmo2774， lmo2775和lmo2776的3'末端部分）。需要进一步研究高度持久的菌株，以确定缺乏这些基因是否会促进持久性。尽管基因组比较并未对持久性表型做出明确的生理解释，但两种菌株之间的显着相似性表明，在食品加工环境中选择了具有特定性状的亚型，并且特定的遗传和生理因素是持久性表型的原因。表型。

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