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首页> 外文期刊>Applied Microbiology >Survival, Sublethal Injury, and Recovery of Environmental Burkholderia pseudomallei in Soil Subjected to Desiccation
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Survival, Sublethal Injury, and Recovery of Environmental Burkholderia pseudomallei in Soil Subjected to Desiccation

机译:干燥环境下土壤假单胞伯克霍尔德氏菌的存活,亚致死性和恢复

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Environmental Burkholderia pseudomallei isolated from sandy soil at Castle Hill, Townsville, in the dry tropic region of Queensland, Australia, was inoculated into sterile-soil laboratory microcosms subjected to variable soil moisture. Survival and sublethal injury of the B. pseudomallei strain were monitored by recovery using culture-based methods. Soil extraction buffer yielded higher recoveries as an extraction agent than sterile distilled water. B. pseudomallei was not recoverable when inoculated into desiccated soil but remained recoverable from moist soil subjected to 91 days' desiccation and showed a growth response to increased soil moisture over at least 113 days. Results indicate that endemic dry tropic soil may act as a reservoir during the dry season, with an increase in cell number and potential for mobilization from soil into water in the wet season.
机译:从澳大利亚昆士兰干旱热带地区汤斯维尔城堡山的沙质土壤中分离出的环境伯克霍尔德菌假单胞菌接种到无菌土壤实验室缩影中,土壤微分变化。使用基于培养物的方法,通过恢复来监测假苹果芽孢杆菌菌株的存活和亚致死损伤。土壤提取缓冲液作为提取剂的回收率高于无菌蒸馏水。假芽孢杆菌在接种到干燥的土壤中后无法恢复,但在经过91天的干燥处理后仍能从潮湿的土壤中恢复,并且在至少113天的时间内表现出对土壤水分增加的生长响应。结果表明,特有的干燥热带土壤可能在干旱季节充当储层,细胞数量增加,在潮湿季节从土壤迁移到水中的潜力更大。

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