Technique for Measuring 14CO2 Uptake by Soil Microorganisms In Situ

David W. Smith; Carl B. Fliermans; Thomas D. Brock

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Technique for Measuring 14CO2 Uptake by Soil Microorganisms In Situ

机译：原位土壤微生物对14CO2吸收的测量技术

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Uptake of 14CO2 in soils due to algae or sulfur-oxidizing bacteria was examined by incubation of soil samples with gaseous 14CO2 and subsequent chemical oxidation of biologically fixed radioactive isotope to 14CO2 for detection with a liquid scintillation counting system. The 14CO2 was added to the soil in the gas phase so that no alteration of the moisture or ionic strength of the soil occurred. Wet oxidation of radioactive organic matter was carried out in sealed ampoules, and the 14CO2 produced was transferred to a phenethylamine-liquid scintillation counting system with a simply constructed apparatus. The technique is inexpensive and efficient and does not require elaborate traps since several possible interfering factors were found to have no harmful effects. Experiments in coal mine regions and in geothermal habitats have demonstrated the ecological applicability of this technique for measurement of CO2 fixation by sulfur-oxidizing bacteria and soil algae.

机译：通过将土壤样品与气态14CO2一起孵育，然后将生物固定的放射性同位素化学氧化为14CO2，以使用液体闪烁计数系统进行检测，来检查藻类或硫氧化细菌在土壤中对14CO2的吸收。将14CO2以气相添加到土壤中，因此不会发生土壤水分或离子强度的变化。在密封的安瓿瓶中进行放射性有机物的湿式氧化，将生成的14CO2用简单的设备转移到苯乙胺-液体闪烁计数系统中。由于发现了几种可能的干扰因素没有有害影响，因此该技术廉价且有效，并且不需要复杂的陷阱。在煤矿地区和地热栖息地进行的实验表明，该技术可用于测量硫氧化细菌和土壤藻类对二氧化碳的固定性。

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《Applied Microbiology》 |1972年第3期|共6页
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David W. Smith; Carl B. Fliermans; Thomas D. Brock;
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中图分类微生物学;
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2. Improved Flotation Technique for Microscopy of In Situ Soil and Sediment Microorganisms [J] . T. L. Bone, D. L. Balkwill Applied and Environmental Microbiology . 1986,第3期

机译：改良的浮选技术用于原位土壤和沉积物微生物的显微镜检查
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5. Limitations and usefulness of nitrogen-15 techniques in measuring mineralization/immobilization and plant uptake of soil and fertilizer nitrogen [D] . Lauren, Julie Gabrielle. 1991

机译：氮15技术在测量土壤和肥料中氮的矿化/固定化和植物吸收方面的局限性和实用性
6. Technique for Measuring 14CO2 Uptake by Soil Microorganisms In Situ [O] . David W. Smith, Carl B. Fliermans, Thomas D. Brock 1972

机译：原位土壤微生物对14CO2吸收的测量技术
7. Technique for Measuring 14CO2 Uptake by Soil Microorganisms In Situ [O] . David W. Smith, Carl B. Fliermans, Thomas D. Brock 1972

机译：原位土壤微生物测量14Co2摄取的技术
8. Improved Flotation Technique for Microscopy of 'In situ' Soil and Sediment Microorganisms [R] . Bone, T. L. , Balkwill, D. L. 1986

机译：改进的“原地”土壤和沉积物微生物显微镜浮选技术

Technique for Measuring 14CO2 Uptake by Soil Microorganisms In Situ

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