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首页> 外文期刊>Applied Microbiology >The Type II Secretion System Is Essential for Erythrocyte Lysis and Gut Colonization by the Leech Digestive Tract Symbiont Aeromonas veronii
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The Type II Secretion System Is Essential for Erythrocyte Lysis and Gut Colonization by the Leech Digestive Tract Symbiont Aeromonas veronii

机译：II型分泌系统是水the消化道共生菌气单胞菌对红细胞裂解和肠道定植所必不可少的

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摘要

Hemolysin and the type II secretion system (T2SS) have been shown to be important for virulence in many pathogens, but very few studies have shown their importance in beneficial microbes. Here, we investigated the importance of the type II secretion pathway in the beneficial digestive-tract association of Aeromonas veronii and the medicinal leech Hirudo verbana and revealed a critical role for the hemolysis of erythrocytes. A mutant with a miniTn 5 insertion in exeM , which is involved in forming the inner membrane platform in the T2SS, was isolated by screening mutants for loss of hemolysis on blood agar plates. A hemolysis assay was used to quantify the mutant's deficiency in lysing sheep erythrocytes and revealed a 99.9% decrease compared to the parent strain. The importance of the T2SS in the colonization of the symbiotic host was assessed. Colonization assays revealed that the T2SS is critical for initial colonization of the leech gut. The defect was tied to the loss of hemolysin production by performing a colonization assay with blood containing lysed erythrocytes. This restored the colonization defect in the mutant. Complementation of the mutant using the promoter region and exeMN revealed that the T2SS is responsible for secreting hemolysin into the extracellular space and that both the T2SS and hemolysin export by the T2SS are critical for initial establishment of A. veronii in the leech gut.
机译：溶血素和II型分泌系统（T2SS）已被证明对许多病原体的毒力很重要，但是很少有研究表明它们对有益微生物的重要性。在这里，我们调查了II型分泌途径在有益的消化道关联的维罗纳气单胞菌和药用水echHirudo verbana的重要性，并揭示了对红细胞溶血的关键作用。通过筛选在血琼脂平板上失去溶血作用的突变体，分离出在exeM中插入miniTn 5的突变体，该突变体参与在T2SS中形成内膜平台。溶血分析用于定量突变体在裂解绵羊红细胞中的缺陷，与亲本菌株相比，降低了99.9％。评估了T2SS在共生宿主定殖中的重要性。定殖试验表明，T2SS对于水the肠道的初始定殖至关重要。通过用含有溶解红细胞的血液进行定植试验，该缺陷与溶血素产生的损失有关。这恢复了突变体中的定植缺陷。使用启动子区域和exeMN对突变体的补充表明，T2SS负责将溶血素分泌到细胞外空间，并且T2SS和T2SS的溶血素输出对于水ver肠中最初建立的拟南芥至关重要。

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