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首页> 外文期刊>Applied Microbiology >Investigations of Salmonella enterica Serovar Newport Infections of Oysters by Using Immunohistochemistry and Knockout Mutagenesis
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Investigations of Salmonella enterica Serovar Newport Infections of Oysters by Using Immunohistochemistry and Knockout Mutagenesis

机译：利用免疫组织化学和基因敲除诱变技术研究牡蛎肠道沙门氏菌血清新港感染

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摘要

The consumption of raw oysters is an important risk factor in the acquisition of food-borne disease, with Salmonella being one of a number of pathogens that have been found in market oysters. Previous work by our lab found that Salmonella was capable of surviving in oysters for over 2 months under laboratory conditions, and this study sought to further investigate Salmonella 's tissue affinity and mechanism of persistence within the oysters. Immunohistochemistry was used to show that Salmonella was capable of breaching the epithelial barriers, infecting the deeper connective tissues of the oysters, and evading destruction by the oysters' phagocytic hemocytes. To further investigate the mechanism of these infections, genes vital to the function of Salmonella 's two main type III secretion systems were disrupted and the survivability of these knockout mutants within oysters was assayed. When the Salmonella pathogenicity island 1 and 2 mutant strains were exposed to oysters, there were no detectable deficiencies in their abilities to survive, suggesting that Salmonella 's long-term infection of oysters does not rely upon these two important pathogenicity islands and must be due to some other, currently unknown, mechanism.
机译：生牡蛎的消费是获得食源性疾病的重要风险因素，沙门氏菌是在市场牡蛎中发现的许多病原体之一。我们实验室先前的工作发现沙门氏菌能够在实验室条件下在牡蛎中存活超过2个月，并且本研究试图进一步研究沙门氏菌的组织亲和力和牡蛎内的持久性机制。免疫组织化学用于显示沙门氏菌能够突破上皮屏障，感染牡蛎的更深结缔组织，并逃避了牡蛎吞噬血细胞的破坏。为了进一步研究这些感染的机制，破坏了对沙门氏菌两个主要III型分泌系统功能至关重要的基因，并分析了这些敲除突变体在牡蛎中的生存能力。当沙门氏菌致病岛1和2突变菌株暴露于牡蛎时，它们的生存能力没有可检测到的缺陷，这表明沙门氏菌对牡蛎的长期感染并不依赖于这两个重要的致病岛，这必须是由于其他一些目前未知的机制。

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