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Supplementation of Intracellular XylR Leads to Coutilization of Hemicellulose Sugars

机译：细胞内XylR的补充导致半纤维素糖的共同利用

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Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.
机译：大肠杆菌有潜力成为强大的生物催化剂，可将木质纤维素生物质转化为有用的材料，例如生物燃料和聚合物。使用大肠杆菌转化生物质糖的一个重要挑战是双生糖或不同类型糖的连续利用。我们证明，通过增加细胞内转录因子XylR的水平，可以消除木糖之前阿拉伯糖的优先消费。另外，必须对XylR的扩增进行微调，以使这两种半纤维素糖具有强大的共利用率。使用一种用于无痕基因插入的新技术，将额外的xylR拷贝插入araBAD操纵子。所得菌株在阿拉伯糖和木糖的混合物代谢方面表现优异，并且能够比野生型菌株多生产至少36％的乙醇。该菌株是开发可同时转化所有生物质糖的大肠杆菌生物催化剂的有用起点。

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