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首页> 外文期刊>Applied Microbiology >TiO2 Photocatalysis Damages Lipids and Proteins in Escherichia coli
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TiO2 Photocatalysis Damages Lipids and Proteins in Escherichia coli

机译：TiO2光催化破坏大肠杆菌中的脂质和蛋白质

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This study investigates the mechanisms of UV-A (315 to 400 nm) photocatalysis with titanium dioxide (TiO_(2)) applied to the degradation of Escherichia coli and their effects on two key cellular components: lipids and proteins. The impact of TiO_(2) photocatalysis on E. coli survival was monitored by counting on agar plate and by assessing lipid peroxidation and performing proteomic analysis. We observed through malondialdehyde quantification that lipid peroxidation occurred during the photocatalytic process, and the addition of superoxide dismutase, which acts as a scavenger of the superoxide anion radical (O_(2)·~(?)), inhibited this effect by half, showing us that O_(2)·~(?) radicals participate in the photocatalytic antimicrobial effect. Qualitative analysis using two-dimensional electrophoresis allowed selection of proteins for which spot modifications were observed during the applied treatments. Two-dimensional electrophoresis highlighted that among the selected protein spots, 7 and 19 spots had already disappeared in the dark in the presence of 0.1 g/liter and 0.4 g/liter TiO_(2), respectively, which is accounted for by the cytotoxic effect of TiO_(2). Exposure to 30 min of UV-A radiation in the presence of 0.1 g/liter and 0.4 g/liter TiO_(2) increased the numbers of missing spots to 14 and 22, respectively. The proteins affected by photocatalytic oxidation were strongly heterogeneous in terms of location and functional category. We identified several porins, proteins implicated in stress response, in transport, and in bacterial metabolism. This study reveals the simultaneous effects of O_(2)·~(?) on lipid peroxidation and on the proteome during photocatalytic treatment and therefore contributes to a better understanding of molecular mechanisms in antibacterial photocatalytic treatment.
机译：这项研究调查了二氧化钛（TiO_（2））的UV-A（315至400 nm）光催化作用机理对大肠杆菌的降解及其对两个关键细胞成分的影响：脂质和蛋白质。 TiO_（2）光催化作用对大肠杆菌存活的影响通过在琼脂平板上计数，评估脂质过氧化作用和进行蛋白质组学分析来监测。我们通过丙二醛定量观察到，在光催化过程中发生了脂质过氧化作用，并且作为超氧化物阴离子自由基（O_（2）·〜（？））的清除剂的超氧化物歧化酶的添加将这种效应抑制了一半，表明我们认为O_（2）·〜（？）自由基参与了光催化抗菌作用。使用二维电泳的定性分析可以选择在应用的处理过程中观察到斑点修饰的蛋白质。二维电泳显示，在选择的蛋白质斑点中，分别存在0.1 g / l和0.4 g / l TiO_（2）的情况下，在黑暗中已经消失了7个斑点和19个斑点，这是由细胞毒性作用引起的的TiO_（2）。在0.1 g /升和0.4 g /升TiO_（2）的存在下暴露于30分钟的UV-A辐射会使缺失点的数量分别增加到14和22。受光催化氧化作用影响的蛋白质在位置和功能类别方面非常异质。我们鉴定了几种孔蛋白，这些蛋白与应激反应，运输和细菌代谢有关。这项研究揭示了O_（2）·〜（？）对光催化处理过程中脂质过氧化和蛋白质组的同时影响，因此有助于更好地理解抗菌光催化处理的分子机理。

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