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Production of Alanine by Fusarium moniliforme

机译：镰孢镰刀菌生产丙氨酸

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Fusarium moniliforme grown in a chemically defined medium in submerged culture accumulated amino acids extracellularly. Alanine and glutamic acid were present in greatest amounts, with traces of glycine, lysine, threonine, and valine detectable. Increasing the glucose and urea concentrations of the medium increased yields of alanine. Further increases in alanine production occurred with elevated levels of mineral salts in the medium, whereas the addition of a vitamin mixture proved to be inhibitory. Chemical changes resulting from the growth of F. moniliforme in the final fermentation medium disclosed maximal alanine production, mycelial weight, and glucose consumption after 72 hr of incubation at 28.5 C. Total soluble nitrogen, by contrast, was minimal at the same time period. The pH remained in the alkaline range throughout the fermentation.
机译：在水下培养中化学成分确定的培养基中生长的镰刀菌镰刀菌会在细胞外积累氨基酸。丙氨酸和谷氨酸的含量最高，可检测到痕量的甘氨酸，赖氨酸，苏氨酸和缬氨酸。增加培养基中的葡萄糖和尿素浓度可提高丙氨酸的产量。随着培养基中矿物质盐含量的增加，丙氨酸的产量进一步增加，而添加维生素混合物被证明具有抑制作用。在28.5 C下孵育72小时后，最终发酵培养基中念珠菌的生长导致的化学变化显示出最大的丙氨酸产量，菌丝重和葡萄糖消耗。相反，总可溶氮在同一时间段内极少。在整个发酵过程中，pH值都保持在碱性范围内。

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