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Preservation of Serratia marcescens by High-Vacuum Lyophilization

机译：高压冻干法保存粘质沙雷氏菌

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Water-washed Serratia marcescens (ATCC strain 14041) cells were lyophilized in an all-glass system capable of evacuation to pressures of less than 5 × 10-6 torr. Lyophilization at the lowest pressures resulted in 50 to 65% survival for unstabilized washed organisms compared with 10 to 20% when the cells were lyophilized at pressures of about 2.5 × 10-2 torr. At the latter pressures, 45 to 65% survival was obtained when NaCl or Naylor-Smith stabilizer was added to the cell suspensions before lyophilization. However, the stabilizers failed to increase significantly the levels of survival compared with water suspension when cells were lyophilized at pressures less than 10-5 torr. The high survival rates obtained by the high-vacuum technique may be attributed to the reduction of traces of molecular oxygen which has been reported to be destructive of the dried bacteria. Survival of unstabilized dried S. marcescens after 1-day storage increased markedly with decreasing sealing pressure. Under the highest vacuum attained, survival of the dried bacteria was not impaired by storage for up to 1 month at Dry Ice temperatures; at higher temperatures, viability losses occurred. Exposure of the dried unstabilized bacteria to dry air resulted in rapid viability loss. The inactivation could be stopped almost immediately by evacuation to pressures of less than 10-5 torr, but the evacuation failed to reverse the viability losses that occurred during exposure.
机译：将水洗的粘质沙雷氏菌（ATCC菌株14041）细胞在能够排空至小于5×10-6托的压力的全玻璃系统中冻干。在最低压力下冻干可导致不稳定的洗涤生物存活50％至65％，而在约2.5×10-2托的压力下冻干细胞则可达到10％至20％。在后者的压力下，冻干前向细胞悬浮液中添加NaCl或Naylor-Smith稳定剂可获得45至65％的存活率。但是，当细胞在小于10-5托的压力下冻干时，与水悬浮液相比，稳定剂无法显着提高存活率。通过高真空技术获得的高存活率可以归因于减少了据称对干燥细菌具有破坏性的痕量分子氧。随着密封压力的降低，未稳定的干燥的马赛菌的生存期为1天，显着增加。在最高真空下，在干冰温度下保存长达1个月不会损害干燥细菌的生存;在较高温度下，会发生活力损失。将干燥的不稳定细菌暴露于干燥空气中会导致迅速丧失活力。通过抽空到小于10-5托的压力，灭活几乎可以立即停止，但是抽空不能逆转在暴露过程中发生的活力损失。

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