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Recovery of Small Quantities of Viruses from Clean Waters on Cellulose Nitrate Membrane Filters

机译：硝酸纤维素膜过滤器从清洁水中回收少量病毒

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摘要

A method is described for quantitatively recovering small amounts of viruses from large volumes of buffered, distilled water. Development of the method was motivated by the anticipated need for testing large volumes of renovated sewage for viruses. The method consists of adsorbing viruses onto cellulose nitrate membrane filters (0.45 μm pore size) from water containing sufficient Na2HPO4 to produce a molarity of 0.05 and sufficient citric acid to produce a pH of 7, and eluting the adsorbed viruses in 3% beef extract under extended sonic treatment. Complete recovery of poliovirus 1, echovirus 7, and coxsackievirus B3 resulted when less than 100 plaque-forming units were added to 1-liter quantities of water. Recoveries of reovirus 1 were almost as good. Preliminary studies indicate that good recoveries can be made from 25-gal quantities of water. The method described is efficient in waters of high quality and may be useful for recovering viruses in renovated, and perhaps in tap waters, but not in waters containing certain organic matter unless that matter is first removed.
机译：描述了一种用于从大量的缓冲蒸馏水中定量回收少量病毒的方法。该方法的开发是出于对测试大量翻新污水中的病毒的预期需求而推动的。该方法包括从病毒中将病毒吸附到硝酸纤维素膜滤膜（孔径为0.45μm）上，该水中含有足够的Na2HPO4以产生0.05的摩尔浓度和足够的柠檬酸以产生pH值为7，并在3％的牛肉提取物中洗脱被吸附的病毒在长期的声波治疗下。当将不足100个噬菌斑形成单位添加到1升水中时，脊髓灰质炎病毒1，回声病毒7和柯萨奇病毒B3会完全回收。呼肠孤病毒1的恢复几乎一样好。初步研究表明，从25加仑的水中可以得到良好的回收率。所描述的方法在高质量水域中是有效的，并且对于在翻新过的，也许在自来水中的病毒恢复可能是有用的，但在包含某些有机物的水中则不是有效的方法，除非先去除该有机物。

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