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Evaluation of Type A Botulinal Toxin Assays that Use Antitoxin to Crystalline Toxin

机译：评价使用抗毒素结晶毒素的A型肉毒毒素测定

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The type A botulinal toxin assay by the reverse passive hemagglutination procedure which uses antitoxin to crystalline toxin was examined for specificity. The analysis was based on the fact that crystalline type A toxin is a complex of neurotoxic protein (Aα) and a nontoxic protein (Aβ). By using these components, obtained in essentially pure forms, it was shown that the antitoxin to crystalline toxin has a significantly higher titer to Aβ than to Aα. When Formalin-treated red blood cells were sensitized with this antitoxin, the antibodies coupled to the cells were, for practical results, only anti-Aβ. When the suspension is reacted with dilutions of type A toxic solutions, the limiting dilutions are determined by Aβ and not by the neurotoxin, which should be the determinant if the assay is to measure toxicity. These observations may be pertinent to the development of serological assays for other botulinal toxin types.
机译：检查了通过反向被动血凝法使用抗毒素对结晶毒素进行的A型肉毒毒素的特异性。该分析基于以下事实：A型结晶毒素是神经毒性蛋白（Aα）和无毒蛋白（Aβ）的复合物。通过使用以基本纯净的形式获得的这些组分，表明对结晶毒素的抗毒素对Aβ的效价明显高于对Aα的效价。当福尔马林处理过的红细胞用这种抗毒素敏化时，为了实际结果，与细胞偶联的抗体仅是抗Aβ。当悬浮液与A型有毒溶液的稀释液反应时，极限稀释液由Aβ决定，而不是由神经毒素决定，如果要测定毒性，则应由神经毒素决定。这些观察结果可能与其他肉毒毒素类型血清学检测方法的发展有关。

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