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首页> 外文期刊>Applied Microbiology >Modified Radioimmunoassay for Murine Sarcoma-Leukemia Virus Group-Specific Antigen
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Modified Radioimmunoassay for Murine Sarcoma-Leukemia Virus Group-Specific Antigen

机译：小鼠肉瘤-白血病病毒组特异性抗原的改良放射免疫分析

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摘要

Iodination of disrupted Moloney strain murine sarcoma-leukemia virus resulted in labeled group-specific (gs) protein which was subsequently purified on an isoelectrofocusing column. This iodinated purified gs antigen, prepared from a relatively small quantity of purified virus, was used in a radioimmunoassay. A radioimmunoassay inhibition method was developed so that antibody specific for mammalian C-type gs antigen could be measured in undiluted or low dilutions of test serum without altering the known reagents of the test. The gs antigen isolated from purified Moloney strain murine sarcoma-leukemia virus has an isoelectric point (pH 5.95) which is significantly lower than that reported for other murine leukemia viruses.
机译：碘化破坏的莫洛尼氏菌鼠肉瘤白血病病毒后，产生标记的组特异性（gs）蛋白，随后将其在等电聚焦柱上纯化。由相对少量的纯化病毒制备的这种碘化的纯化gs抗原用于放射免疫测定中。开发了一种放射免疫测定抑制方法，以便可以在未稀释或低稀释的测试血清中测量对哺乳动物C型gs抗原具有特异性的抗体，而无需更改测试的已知试剂。从纯化的莫洛尼氏菌鼠肉瘤白血病病毒中分离出的gs抗原的等电点（pH 5.95）明显低于其他鼠类白血病病毒的报道。

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