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首页> 外文期刊>Applied Microbiology >Comparative Analysis of Acidobacterial Genomic Fragments from Terrestrial and Aquatic Metagenomic Libraries, with Emphasis on Acidobacteria Subdivision 6
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Comparative Analysis of Acidobacterial Genomic Fragments from Terrestrial and Aquatic Metagenomic Libraries, with Emphasis on Acidobacteria Subdivision 6

机译：陆生和水生基因组学图书馆酸细菌基因组片段的比较分析，重点是酸细菌细分6

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The bacterial phylum Acidobacteria has a widespread distribution and is one of the most common and diverse phyla in soil habitats. However, members of this phylum have often been recalcitrant to cultivation methods, hampering the study of this presumably important bacterial group. In this study, we used a cultivation-independent metagenomic approach to recover genomic information from soilborne members of this phylum. A soil metagenomic fosmid library was screened by PCR targeting acidobacterial 16S rRNA genes, facilitating the recovery of 17 positive clones. Recovered inserts appeared to originate from a range of Acidobacteria subdivisions, with dominance of subdivision 6 (10 clones). Upon full-length insert sequencing, gene annotation identified a total of 350 open reading frames (ORFs), representing a broad range of functions. Remarkably, six inserts from subdivision 6 contained a region of gene synteny, containing genes involved in purine de novo biosynthesis and encoding tRNA synthetase and conserved hypothetical proteins. Similar genomic regions had previously been observed in several environmental clones recovered from soil and marine sediments, facilitating comparisons with respect to gene organization and evolution. Comparative analyses revealed a general dichotomy between marine and terrestrial genes in both phylogeny and G+C content. Although the significance of this homologous gene cluster across subdivision 6 members is not known, it appears to be a common feature within a large percentage of all acidobacterial genomic fragments recovered from both of these environments.
机译：细菌门酸性细菌分布广泛，是土壤生境中最常见和最多样化的门之一。但是，该门的成员通常对培养方法不满意，从而妨碍了对这一可能重要的细菌群的研究。在这项研究中，我们使用了一种与栽培无关的宏基因组学方法，从该门的土壤传播成员中恢复了基因组信息。通过针对酸性细菌16S rRNA基因的PCR筛选了土壤宏基因组化的fosmid文库，促进了17个阳性克隆的回收。回收的插入片段似乎起源于一系列酸性细菌亚种，占主导地位的是亚6类（10个克隆）。通过全长插入测序，基因注释识别出总共350个开放阅读框（ORF），代表了广泛的功能。值得注意的是，来自第6分区的六个插入片段包含一个基因同构区域，其中包含与嘌呤从头生物合成有关的基因，并编码tRNA合成酶和保守的假设蛋白质。以前在从土壤和海洋沉积物中回收的几个环境克隆中都观察到了相似的基因组区域，这有助于在基因组织和进化方面进行比较。比较分析显示，海洋和陆地基因在系统发育和G + C含量上普遍存在二分法。尽管该同源基因簇在第6分区成员中的重要性尚不清楚，但这似乎是从这两种环境中回收的所有酸性细菌基因组片段中很大一部分的共同特征。

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