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Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries
Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries
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机译：促进象间DNA中的功能性DNA表达的转座子和在偏离的方法分析期间增强DNA转录的方法
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摘要
A transposon (TnC_T7) was developed to partially supply the transcriptional machinery during functional analysis of genomic/metagenomic libraries. This transposon was conceived and constructed to have the ability to integrate randomly into any episomal DNA, allowing the inducible expression of the adjacent DNA regions in both directions. In general, this genetic tool included a kanamycin resistance gene, two bidirectional T7 promoters and the T7RNA polymerase-coding gene, the latter under the regulation of the inducible arabinose promoter (PBAD). The experimental validation confirmed the TnC_T7 potential to be used in functional genomic/metagenomic studies, in order to partially overcome the limitations of the bacterial hosts, which prevent them to recognize most of foreign genes from DNA libraries.
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