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首页> 外文期刊>Applied Microbiology >Mercury and Other Heavy Metals Influence Bacterial Community Structure in Contaminated Tennessee Streams
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Mercury and Other Heavy Metals Influence Bacterial Community Structure in Contaminated Tennessee Streams

机译：汞和其他重金属影响受污染的田纳西州河流中的细菌群落结构

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High concentrations of uranium, inorganic mercury [Hg(II)], and methylmercury (MeHg) have been detected in streams located in the Department of Energy reservation in Oak Ridge, TN. To determine the potential effects of the surface water contamination on the microbial community composition, surface stream sediments were collected 7 times during the year, from 5 contaminated locations and 1 control stream. Fifty-nine samples were analyzed for bacterial community composition and geochemistry. Community characterization was based on GS 454 FLX pyrosequencing with 235 Mb of 16S rRNA gene sequence targeting the V4 region. Sorting and filtering of the raw reads resulted in 588,699 high-quality sequences with lengths of >200 bp. The bacterial community consisted of 23 phyla, including Proteobacteria (ranging from 22.9 to 58.5% per sample), Cyanobacteria (0.2 to 32.0%), Acidobacteria (1.6 to 30.6%), Verrucomicrobia (3.4 to 31.0%), and unclassified bacteria. Redundancy analysis indicated no significant differences in the bacterial community structure between midchannel and near-bank samples. Significant correlations were found between the bacterial community and seasonal as well as geochemical factors. Furthermore, several community members within the Proteobacteria group that includes sulfate-reducing bacteria and within the Verrucomicrobia group appeared to be associated positively with Hg and MeHg. This study is the first to indicate an influence of MeHg on the in situ microbial community and suggests possible roles of these bacteria in the Hg/MeHg cycle.
机译：在田纳西州橡树岭能源部保留地中发现了高浓度的铀，无机汞[Hg（II）]和甲基汞（MeHg）。为了确定地表水污染对微生物群落组成的潜在影响，一年中从5个受污染的地点和1个对照流中收集了7次地表流沉积物。分析了59个样品的细菌群落组成和地球化学。社区表征基于GS 454 FLX焦磷酸测序，靶向235 Vb的16S rRNA基因序列靶向V4区。原始读数的分类和过滤产生了588,699个高质量序列，长度大于200 bp。细菌群落由23个门组成，包括变形杆菌（每个样品的22.9％至58.5％），蓝细菌（0.2％至32.0％），酸性细菌（1.6％至30.6％），疣状微生物菌（3.4％至31.0％）和未分类的细菌。冗余分析表明，中通道和近岸样品之间的细菌群落结构没有显着差异。在细菌群落与季节和地球化学因素之间发现了显着的相关性。此外，Proteobacteria组中的一些社区成员（包括减少硫酸盐的细菌）和Verrucomicrobia组中的一些社区成员似乎与Hg和MeHg呈正相关。这项研究首次表明了MeHg对原位微生物群落的影响，并暗示了这些细菌在Hg / MeHg循环中的可能作用。

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