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首页> 外文期刊>Applied Microbiology >Denaturing Gradient Gel Electrophoresis and Barcoded Pyrosequencing Reveal Unprecedented Archaeal Diversity in Mangrove Sediment and Rhizosphere Samples
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Denaturing Gradient Gel Electrophoresis and Barcoded Pyrosequencing Reveal Unprecedented Archaeal Diversity in Mangrove Sediment and Rhizosphere Samples

机译：变性梯度凝胶电泳和条形码焦磷酸测序揭示了红树林沉积物和根际样品中前所未有的古细菌多样性

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Mangroves are complex ecosystems that regulate nutrient and sediment fluxes to the open sea. The importance of bacteria and fungi in regulating nutrient cycles has led to an interest in their diversity and composition in mangroves. However, very few studies have assessed Archaea in mangroves, and virtually nothing is known about whether mangrove rhizospheres affect archaeal diversity and composition. Here, we studied the diversity and composition of Archaea in mangrove bulk sediment and the rhizospheres of two mangrove trees, Rhizophora mangle and Laguncularia racemosa , using denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of archaeal 16S rRNA genes with a nested-amplification approach. DGGE profiles revealed significant structural differences between bulk sediment and rhizosphere samples, suggesting that roots of both mangrove species influence the sediment archaeal community. Nearly all of the detected sequences obtained with pyrosequencing were identified as Archaea , but most were unclassified at the level of phylum or below. Archaeal richness was, furthermore, the highest in the L. racemosa rhizosphere, intermediate in bulk sediment, and the lowest in the R. mangle rhizosphere. This study shows that rhizosphere microhabitats of R. mangle and L. racemosa , common plants in subtropical mangroves located in Rio de Janeiro, Brazil, hosted distinct archaeal assemblages.
机译：红树林是复杂的生态系统，可调节营养物质和沉积物通向公海的流量。细菌和真菌在调节养分循环中的重要性已引起人们对红树林中生物多样性和组成的兴趣。但是，很少有研究评估红树林中的古生菌，而关于红树林的根际是否影响古细菌的多样性和组成却几乎一无所知。在这里，我们使用变性梯度凝胶电泳（DGGE）和古细菌16S rRNA基因的焦磷酸测序和巢式扩增方法研究了红树林块状沉积物中古生菌和两种红树林树根际（Rhizophora mangle和Laguncularia racemosa）的根际的多样性和组成。 DGGE剖面揭示了散装沉积物和根际样品之间的显着结构差异，表明这两种红树林物种的根都影响着沉积物古细菌群落。通过焦磷酸测序获得的几乎所有检测到的序列都被鉴定为古细菌，但大多数未分类到门或低于门的水平。此外，古生菌的丰富度在racemosa根际中最高，在大量沉积物中居中，而在mangle根际中最低。这项研究表明，位于巴西里约热内卢的亚热带红树林的常见植物R. mangle和L. racemosa的根际微生境具有独特的古细菌组合。

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