...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Two-Year Systematic Study To Assess Norovirus Contamination in Oysters from Commercial Harvesting Areas in the United Kingdom
【24h】

Two-Year Systematic Study To Assess Norovirus Contamination in Oysters from Commercial Harvesting Areas in the United Kingdom

机译：一项为期两年的系统研究，旨在评估英国商业采伐区牡蛎中诺如病毒的污染

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The contamination of bivalve shellfish with norovirus from human fecal sources is recognized as an important human health risk. Standardized quantitative methods for the detection of norovirus in molluscan shellfish are now available, and viral standards are being considered in the European Union and internationally. This 2-year systematic study aimed to investigate the impact of the application of these methods to the monitoring of norovirus contamination in oyster production areas in the United Kingdom. Twenty-four monthly samples of oysters from 39 United Kingdom production areas, chosen to represent a range of potential contamination risk, were tested for norovirus genogroups I and II by using a quantitative real-time reverse transcription (RT)-PCR method. Norovirus was detected in 76.2% (643/844) of samples, with all sites returning at least one positive result. Both prevalences (presence or absence) and norovirus levels varied markedly between sites. However, overall, a marked winter seasonality of contamination by both prevalence and quantity was observed. Correlations were found between norovirus contamination and potential risk indicators, including harvesting area classifications, Escherichia coli scores, and environmental temperatures. A predictive risk score for norovirus contamination was developed by using a combination of these factors. In , this study, the largest of its type undertaken to date, provides a systematic analysis of norovirus contamination in commercial oyster production areas in the United Kingdom. The data should assist risk managers to develop control strategies to reduce the risk of human illness resulting from norovirus contamination of bivalve molluscs.
机译：人类粪便来源的诺如病毒对双壳贝类的污染被认为是重要的人类健康风险。现在已经有了用于检测软体动物贝类中诺如病毒的标准化定量方法，并且欧盟和国际上都在考虑病毒标准。这项为期2年的系统研究旨在调查这些方法的应用对英国牡蛎产区诺如病毒污染监测的影响。通过使用定量实时逆转录（RT）-PCR方法对来自英国39个生产地区的24个每月牡蛎样本进行了诺如病毒I和II型基因组测试，这些样本被选为代表一定范围的潜在污染风险。在76.2％（643/844）的样本中检测到诺如病毒，所有位点均返回至少一个阳性结果。患病率（存在或不存在）和诺如病毒水平在站点之间存在显着差异。但是，总的来说，观察到冬季的流行率和数量均显示出明显的冬季污染季节。发现诺如病毒污染与潜在风险指标之间存在相关性，包括收获区分类，大肠杆菌得分和环境温度。通过结合使用这些因素，得出了诺如病毒污染的预测风险评分。在中，这项研究是迄今为止进行的最大规模的研究，它对英国商业牡蛎产区的诺如病毒污染进行了系统分析。数据应有助于风险管理者制定控制策略，以减少诺如病毒对双壳贝类软体动物的污染所致的人类疾病风险。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号