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首页> 外文期刊>Applied Microbiology >Survival and Germination of Bacillus cereus Spores without Outgrowth or Enterotoxin Production during In Vitro Simulation of Gastrointestinal Transit
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Survival and Germination of Bacillus cereus Spores without Outgrowth or Enterotoxin Production during In Vitro Simulation of Gastrointestinal Transit

机译：胃肠道过境体外模拟过程中无增生或肠毒素产生的蜡状芽孢杆菌孢子的存活和萌发

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摘要

To study the gastrointestinal survival and enterotoxin production of the food-borne pathogen Bacillus cereus , an in vitro simulation experiment was developed to mimic gastrointestinal passage in 5 phases: (i) the mouth, (ii) the stomach, with gradual pH decrease and fractional emptying, (iii) the duodenum, with high concentrations of bile and digestive enzymes, (iv) dialysis to ensure bile reabsorption, and (v) the ileum, with competing human intestinal bacteria. Four different B. cereus strains were cultivated and sporulated in mashed potato medium to obtain an inoculum of 7.0 log spores/ml. The spores showed survival and germination during the in vitro simulation of gastrointestinal passage, but vegetative outgrowth of the spores was suppressed by the intestinal bacteria during the final ileum phase. No bacterial proliferation or enterotoxin production was observed, despite the high inoculum levels. Little strain variability was observed: except for the psychrotrophic food isolate, the spores of all strains survived well throughout the gastrointestinal passage. The in vitro simulation experiments investigated the survival and enterotoxin production of B. cereus in the gastrointestinal lumen. The results obtained support the hypothesis that localized interaction of B. cereus with the host's epithelium is required for diarrheal food poisoning.
机译：为了研究食源性蜡状芽孢杆菌的胃肠道存活和肠毒素产生，开展了一个体外模拟实验，以模拟胃肠道通过5个阶段的过程：（i）口腔，（ii）胃，pH逐渐降低并逐渐降低排空（iii）具有高浓度的胆汁和消化酶的十二指肠，（iv）透析以确保胆汁重吸收，以及（v）与竞争的人类肠道细菌一起回肠。在土豆泥培养基中培养和孢子形成四种不同的蜡状芽孢杆菌菌株，以获得7.0 log孢子/ ml的接种物。在体外胃肠道模拟中，孢子表现出存活和发芽，但是在最后回肠阶段，肠细菌抑制了孢子的营养生长。尽管接种量很高，但未观察到细菌增殖或肠毒素产生。几乎没有观察到菌株的变异性：除精神营养食品分离株外，所有菌株的孢子在整个胃肠道中都存活良好。体外模拟实验研究了蜡状芽孢杆菌在胃肠腔中的存活和肠毒素产生。获得的结果支持以下假设：腹泻食物中毒需要蜡状芽孢杆菌与宿主上皮的局部相互作用。

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