Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria

F. Heath Damron; Elizabeth S. McKenney; Herbert P. Schweizer; Joanna B. Goldberg

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Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria

机译：单拷贝PBAD控制基因在革兰氏阴性细菌中的广泛宿主范围基于Tn7的载体的构建。

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We describe a mini-Tn 7 -based broad-host-range expression cassette for arabinose-inducible gene expression from the P_(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.

机译：我们描述了一个基于迷你Tn 7的广泛宿主表达盒，用于从P_（BAD）启动子进行阿拉伯糖诱导的基因表达。该传递载体pTJ1可以将一个基因的单个拷贝整合到革兰氏阴性细菌的染色体中，以用于多种遗传应用，其中以铜绿假单胞菌为模型宿主，对其中的几种进行了讨论。

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《Applied Microbiology》 |2013年第2期|共4页
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F. Heath Damron; Elizabeth S. McKenney; Herbert P. Schweizer; Joanna B. Goldberg;
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1. Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria [J] . F. Heath Damron, Elizabeth S. McKenney, Herbert P. Schweizer, Applied and Environmental Microbiology . 2013,第2期

机译：单拷贝PBAD控制基因在革兰氏阴性细菌中的广泛宿主范围基于Tn7的载体的构建。
2. Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria [J] . F. Heath Damron, Elizabeth S. McKenney, Herbert P. Schweizer, Applied and Environmental Microbiology . 2013,第2期

机译：单拷贝PBAD控制基因在革兰氏阴性细菌中的广泛宿主范围基于Tn7的载体的构建。
3. IMPROVED BROAD-HOST-RANGE RK2 VECTORS USEFUL FOR HIGH AND LOW REGULATED GENE EXPRESSION LEVELS IN GRAM-NEGATIVE BACTERIA [J] . Blatny JM., Wintherlarsen HC., Karunakaran P., Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 1997,第1期

机译：改良的广谱寄主RK2矢量，可用于革兰氏阴性细菌的高和低调控基因表达水平
4. Predicting Protein Subcellular Localizations for Gram-Negative Bacteria Using DP-PSSM and Support Vector Machines [C] . Juan E.Y.T., Li W.J., Jhang J.H., Complex, Intelligent and Software Intensive Systems, 2009. CISIS '09 . 2009

机译：使用DP-PSSM和支持向量机预测革兰氏阴性细菌的蛋白质亚细胞定位
5. Construction of a Modular Vector Library for varied gene expression in Escherichia coli [D] . Mhatre, Siddhita 2010

机译：大肠杆菌中多种基因表达的模块化载体文库的构建
6. Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria [O] . F. Heath Damron, Elizabeth S. McKenney, Herbert P. Schweizer, 2013

机译：单拷贝PBAD控制基因在革兰氏阴性细菌中的广泛宿主范围基于Tn7的载体的构建。
7. Construction of Mobilizable Mini-Tn7Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy PromoterluxReporter Analysis [O] . F. Heath Damron, Elizabeth S. McKenney, Mariette Barbier, 2013

机译：用于革兰阴影检测的可达迷你TN5的施工和单拷贝促进剂分析

Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria

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